Molecular and General Genetics MGG

, Volume 217, Issue 2–3, pp 471–473

Cloning of the gene coding for chitobiase ofSerratia marcescens

  • Hadar Kless
  • Yaron Sitrit
  • Ilan Chet
  • Amos B. Oppenheim
Article
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Summary

A λ phage DNA library ofSerratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase.Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growingE. coli cells the chitobiase enzyme was found to be secreted into the periplasm.

Key words

Serratia marcescens Chitobiase 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Hadar Kless
    • 1
  • Yaron Sitrit
    • 2
  • Ilan Chet
    • 1
  • Amos B. Oppenheim
    • 2
  1. 1.The Otto Warburg Center for Biotechnology, Faculty of AgricultureHebrew UniversityRehovotIsrael
  2. 2.Department of Molecular GeneticsHebrew University-Hadassah Medical SchoolJerusalemIsrael

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