Molecular and General Genetics MGG

, Volume 217, Issue 2–3, pp 430–436 | Cite as

Cloning, characterization and DNA sequencing of the gene encoding the Mr50000 quinoprotein glucose dehydrogenase fromAcinetobacter calcoaceticus

  • Anne-Marie Cleton-Jansen
  • Nora Goosen
  • Kees Vink
  • Pieter van de Putte


Recently we described the cloning of the gene coding for a Mr 87000 glucose dehydrogenase (GDH-A) fromAcinetobacter calcoaceticus. In this report we describe the cloning of a gene coding for a second GDH (GDH-B) with a Mr of 50000 from the same organism. This gene was isolated using a 20-mer synthetic oligonucleotide, derived from the N-terminal amino acid sequence of purified GDH-B as a probe to screen a genomic bank. From the DNA sequence of thegdhB gene, a protein can be derived of Mr 52772 with a 24 amino acid signal peptide which is removed, resulting in the mature protein with a Mr 50231. In vitro transcription-translation of thegdhB clone shows the mature and the precursor protien. The derived amino acid sequence has no obvious homology with GDH-A ofA. calcoaceticus. We show that disaccharides are specific GDH-B substrates and that 2-deoxyglucose is specific for GDH-A.

Key words

Acinetobacter calcoaceticus DNA sequence Glucose dehydrogenase Pyrroloquinoline quinone Signal sequence 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Anne-Marie Cleton-Jansen
    • 1
  • Nora Goosen
    • 1
  • Kees Vink
    • 1
  • Pieter van de Putte
    • 1
  1. 1.Laboratory of Molecular GeneticsUniversity of LeidenLeidenThe Netherlands

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