, Volume 42, Issue 5, pp 447–453 | Cite as

Ribosomal DNA-ITS sequence polymorphism in the sugarcane rust,Puccinia kuehnii

  • Eric V. Virtudazo
  • Hitoshi Nakamura
  • Makoto Kakishima
Original Papers


The two highly divergent ITS types previously observed in isolates ofPuccinia kuehnii were amplified from the same isolates through the use of ITS type-specific primers for PCR and are therefore considered as polymorphisms of ITS regions within the species. Although homology of sequences of one of the ITS types with otherPuccinia species was of expected levels, significantly high homology of sequences of the other type with those ofCronartium members indicates that abnormal genetic events led to the occurrence of these polymorphic regions. These results indicate that ITS gene tree phylogeny may not reflect true species phylogeny in this group of rust fungi. Rather, D1/D2 region tree phylogeny, which was concordant with the differences in morphology with and among related rusts, more correctly reflects phylogenetic relationships of sugarcane and related grass rusts.

Key Words

Puccinia kuehnii ribosomal DNA ITS polymorphism sugarcane rust 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. Antoniolli, Z. I., Schachtman, D. P., Ophel-Keller, K. and Smith, S. E. 2000. Variation in rDNA ITS sequences inGlomus mossaea andGigaspora margarita spores from a permanent pasture. Mycol. Res.104: 708–715.CrossRefGoogle Scholar
  2. Baldwin, B. G., Sanderson, M. J., Porter, J. M., Wojciechowski, M. F., Campbell, C. S. and Donoghue, M. J. 1995. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann. Missouri Bot. Gard.82: 247–277.CrossRefGoogle Scholar
  3. Bruns, T. D., White, T. J. and Taylor, J. W. 1991. Fungal Molecular Systematics. Annu. Rev. Ecol. Syst.22: 525–564.CrossRefGoogle Scholar
  4. Cummins, G. B. and Hiratsuka, Y. 1983. Illustrated genera of rust fungi, revised ed. The American Phytopathological Society, Minnesota.Google Scholar
  5. Doyle, J. J. 1992. Gene trees and species trees: molecular systematics as one-character state taxonomy. Syst. Bot.17: 144–163.CrossRefGoogle Scholar
  6. Dubcovsky, J. and Dvorak, J. 1995. Ribosomal RNA multigene loci: nomads of the Triticeae genomes. Genetics140: 1367–1377.PubMedGoogle Scholar
  7. Fatehi, J. and Bridge, P. 1998. Detection of multiple rRNA-ITS regions in isolates ofAscochyta. Mycol. Res.102: 762–766.CrossRefGoogle Scholar
  8. Graur, D. and Li W-H. 2000. Fundamentals of Molecular Evolution. 2nd ed. Sinauer Associates, Inc. Massachusetts.Google Scholar
  9. Harlton, C. E., Levesque, C. A. and Punja, Z. K. 1995. Genetic diversity inSclerotium (Athelia) rolfsii and related species. Phytopathology85: 1269–1281.Google Scholar
  10. Hibbet, D. S. 1992. Ribosomal RNA and fungal systematics. Trans. Mycol. Soc. Japan33: 533–556.Google Scholar
  11. Hijri, M., Hosnen, M., van Tuinen, D. and Dulieu, H. 1999. Intraspecific ITS polymorphism inScutellospora castanea (Glomales, Zygomycota) is structured within multinucleate spores. Fung. Gen. Biol.26: 141–151.CrossRefGoogle Scholar
  12. Hillis, D. M. and Davis, S. K. 1988. Ribosomal DNA: intraspecific polymorphism, concerted evolution, and phylogeny reconstruction. Syst. Zool.37: 63–66.CrossRefGoogle Scholar
  13. Hillis, D. M. and Dixon, M. T. 1991. Ribosomal DNA: molecular evolution and phylogenetic inference. Quart. Rev. Biol.64: 411–453.CrossRefGoogle Scholar
  14. Hiratsuka, N., Sato, S., Kakishima, M., Kaneko, S., Sato, T., Hiratsuka, T., Katsuya, K., Hiratsuka, Y., Ono, Y., Harada, Y. and Nakayama, K. 1992. The rust flora of Japan. Tsukuba Shuppankai, Ibaraki.Google Scholar
  15. Hiratsuka, Y. 1988. Ontogeny and morphology of teliospores (probasidia) in Uredinales and their significance in taxonomy and phylogeny. Mycotaxon31: 517–531.Google Scholar
  16. Hiratsuka, Y. and Cummins, G. B. 1963. Morphology of spermonogia of the rust fungi. Mycologia55: 487–507.Google Scholar
  17. Li, W-H. 1997. Molecular Evolution. Sinauer Associates, Inc. Massachusetts.Google Scholar
  18. O’Donnell, K. and Cigelnik, E. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungusFusarium are nonorthologous. Mol. Phylogen. Evol.7: 103–116.CrossRefGoogle Scholar
  19. Page, R. D. and Holmes, E. C. 1998. Molecular evolution: a phylogenetic approach. Blackwell Science Ltd, Oxford.Google Scholar
  20. Sanders, I. R., Alt, M., Groppe, K., Boller, T. and Wiemken, A. 1995. Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytol.130: 419–427.CrossRefGoogle Scholar
  21. Sanderson, M. J. and Doyle, J. J. 1992. Reconstruction of organismal and gene phylogenies from data on multigene families: concerted evolution, homoplasy, and confidence. Syst. Biol.41: 4–17.CrossRefGoogle Scholar
  22. Sjamsuridzal, W., Nishida, H., Ogawa, H., Kakishima, M. and Sugiyama, J. 1999. Phylogenetic relationships of rust fungi parasitic on ferns: Evidence from 18S rDNA sequence analysis. Mycoscience40: 21–27.Google Scholar
  23. Suh, Y., Thien, L. B., Reeve, H. E. and Zimmer, E. A. 1993. Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA in Winteraceae. Am. J. Bot.80: 1042–1055.CrossRefGoogle Scholar
  24. Virtudazo, E. V., Nakamura, H. and Kakishima, M. 2001a. Phylogenetic analysis of sugarcane rusts based on sequences of ITS, 5.8S rDNA and D1/D2 region of LSU rDNA. J. Gen. Plant Pathol.67: 28–36.CrossRefGoogle Scholar
  25. Virtudazo, E. V., Nojima, H. and Kakishima, M. 2001b. Taxonomy ofPuccinia species causing rust diseases on sugarcane. Mycoscience42: 167–175.Google Scholar
  26. Vogler, A. P. and DeSalle, R. 1994. Evolution and phylogenetic information content of the ITS-1 region in the tiger beetleCicindela dorsalis. Mol. Biol. Evol.11: 393–405.PubMedGoogle Scholar
  27. Wagner, A., Blackstone, N., Cartwright, P., Dick, M., Misof, B., Snow, P., Wagner, G. P., Bartels, J., Murtha, M. and Pendleton, J. 1994. Surveys of gene families using polymerase chain reaction: PCR selection and PCR drift. Syst. Biol.43: 250–261.CrossRefGoogle Scholar
  28. Wendel, J. F., Schnabel, A. and Seelanan, T. 1995. Bidirectional interlocus concerted evolution following allopolyploid speciation in cotton (Gossypium). Proc. Natl. Acad. Sci. USA92: 280–284.PubMedCrossRefGoogle Scholar
  29. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1995. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics In: PCR protocols (Innis, M. A., Gelfrand, D. H., Sninsky, J. J. and White, J., eds.), pp. 315–322. Academic Press, San Diego, California.Google Scholar
  30. Zijstra, C., Lever, A. E. M., Uenk, B. J. and Van Silfhuot, C. H. 1995. Differences between ITS regions of isolates of root-knot nematodesMeloidogyne hapla andM. chitwoodi. Phytopathology85: 1231–1237.Google Scholar

Copyright information

© The Mycological Society of Japan 2001

Authors and Affiliations

  • Eric V. Virtudazo
    • 1
  • Hitoshi Nakamura
    • 2
  • Makoto Kakishima
    • 1
  1. 1.Institute of Agriculture and ForestryUniversity of TsukubaTsukuba, IbarakiJapan
  2. 2.National Institute of Agro-Environmental SciencesTsukuba, IbarakiJapan

Personalised recommendations