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Molecular and General Genetics MGG

, Volume 248, Issue 1, pp 43–51 | Cite as

Genes encoding small GTP-binding proteins analogous to mammalian rac are preferentially expressed in developing cotton fibers

  • Deborah P. Delmer
  • Julie R. Pear
  • Andrawis Andrawis
  • David M. Stalker
Original Paper

Abstract

In animals, the small GTP-binding proteins, Rac and Rho, of theras superfamily participate in the signal rransduction pathway that regulates the organization of the actin cytoskeleton. We report here on the characterization of two distinct cDNA clones isolated from a cotton fiber cDNA library that code for homologs of animal Rac proteins. Using gene-specific probes, we have determined that amphidiploid cotton contains two genes that code for each of the two Rac proteins, designated Rac13 and Rac9, respectively. The gene for Rac13 shows highly enhanced expression in developing cotton fibers, with maximal expression occurring at the time of transition between primary and secondary wall synthesis. This is also the time at which reorganization of the cytoskeleton occurs, and thus the pattern of expression of Rac13 is consistent with its possible role, analogous to animal Rac, in the signal transduction pathway that controls cytoskeletal organization.

Key words

Gossypium hirsutum GTP-binding proteins Rac Cotton fiber 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Deborah P. Delmer
    • 1
  • Julie R. Pear
    • 2
  • Andrawis Andrawis
    • 1
  • David M. Stalker
    • 2
  1. 1.Department of Botany, Institute of Life SciencesThe Hebrew University of JerusalemJerusalemIsrael
  2. 2.Calgene Inc.DavisUSA

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