Immobilization and long-term albumin secretion of hepatocyte spheroids rapidly formed by rotational tissue culture methods
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Spheroidal aggregates (spheroids) of adult rat hepatocytes in primary culture formed by rotational tissue culture methods. The time suitable for terminating the rotation and reseeding was determined. The spheroids formed were immobilized stably onto polylysine-coated surfaces, and they secreted albumin for about 2 weeks at almost the same level as compared to spheroids formed by stationary culture.
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