Bulletin of Experimental Biology and Medicine

, Volume 122, Issue 5, pp 1115–1119 | Cite as

Antigen expressed alternatively to α-fetoprotein of rat hepatomas

  • T. L. Eraizer
  • A. K. Yazova
  • V. S. Poltoranina
  • N. I. Kuprina
  • L. Ya. Shipova
  • A. B. Lunkina
  • N. L. Lazarevich
  • G. I. Abelev
Microbiology and Immunology
Received:
  • 18 Downloads

Abstract

Rat hepatoma cell line McA Rh 7777 produces α-fetoprotein (AFP)in vitro. This line is subcloned into ATP-producing and nonproducing subclones. The AFP-negative clone is used to generate hybridomas producing monoclonal antibodies recognizing AFT− and AFP+ clones. One of these hybridomas (A2/3) reacts only with AFP− clones. Double staining for AFP and A2/3 antigen (AG A2/3) reveals alternative expression of AFP and AG A2/3 on McA Rh 7777 hepatoma and its clones. The antigen is present on transplantable H-27 hepatoma (AFP−) and is not expressed by Zaidel hepatoma cells (AFP+). AG A2/3 is absent from the liver of normal adult rats and rat embryos. The antigen is inducedin vivo by the injection of lead nitrate andin vitro by treatment of McA RH 7777 hepatoma and its clones with cadmium chloride. It is likely that AG A2/3 is a cell stress protein.

Key Words

α-fetoprotein hepatoma antigen expressed alternatively to α-fetoprotein 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    G. I. Abelev, in:Tumors as a Problem of Developmental Biology [in Russian], Moscow (1979), pp. 148–169.Google Scholar
  2. 2.
    G. I. Abelev,Immunologiya, No. 3, 4–9 (1994).Google Scholar
  3. 3.
    G. I. Abelev, D. A. El'gort, and T. L. Eraizer,Byull. Eksp. Biol. Med.,92, No. 9, 333–335 (1981).Google Scholar
  4. 4.
    G. A. Beliskii, L. M. Fonshtein, V. V. Khudolei,et al., Eksper. Onkol.,9, No. 3, 20–23 (1987).Google Scholar
  5. 5.
    A. Yu. Kolyada,Biol. Membrany,4, No. 4, 408–414 (1987).Google Scholar
  6. 6.
    E. V. Lekhttsind and A. E. Gurvich,Byull. Eksp. Biol. Med.,92, No. 12, 752–754 (1981).Google Scholar
  7. 7.
    I. N. Shvemberger, in:Cellular Heredity and Malignant Growth [in Russian], Moscow-Leningrad (1966), pp. 154–167.Google Scholar
  8. 8.
    J. Becker, B. de Nechaud, and V. R. Potter,Onco-Developmental Gene Expression, W. H. Fishman and S. Sell (Eds.), New York (1976), pp. 259–270.Google Scholar
  9. 9.
    K. L. Davidson and P. S. Gerald,Methods Cell Biol.,15, 325–338 (1977).PubMedCrossRefGoogle Scholar
  10. 10.
    T. L. Eraiser and L. S. Khamzina,Int. J. Cancer,42, 633–637 (1988).PubMedGoogle Scholar
  11. 11.
    M. W. Roomi and A. Columbano,Carcinogenesis,7, No. 10, 1643–1646 (1986).PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • T. L. Eraizer
    • 1
  • A. K. Yazova
    • 1
  • V. S. Poltoranina
    • 1
  • N. I. Kuprina
    • 1
  • L. Ya. Shipova
    • 1
  • A. B. Lunkina
    • 1
  • N. L. Lazarevich
    • 1
  • G. I. Abelev
    • 1
  1. 1.Institute of Carcinogenesis, Oncology Research CenterRussian Academy of Medical SciencesMoscow

Personalised recommendations