Biotechnology Techniques

, Volume 6, Issue 5, pp 399–404 | Cite as

Characterization of microbial membranes used for the estimation of biochemical oxygen demand with a biosensor

  • E. Galindo
  • J. L. García
  • L. G. Torres
  • R. Quintero


A bacterial mixed culture was immobilized in Millipore filters to construct microbial-membranes for BOD determination using an oxygen electrode. The biosensor response was best when 0.5 mg cells were immobilized per cm2 of membrane, at 30°C, pH 7 and 0.05 M phosphate buffer. Reproducible microbial-membranes can be constructed and they can be stored for up to 20 days without appreciable loss of their response characteristics.


Biochemical Oxygen Demand Millipore Filter Basal Current Current Difference Bacterial Mixed Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • E. Galindo
    • 1
  • J. L. García
    • 1
  • L. G. Torres
    • 1
  • R. Quintero
    • 1
  1. 1.Departmento de Bioingeniería, Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavaca, Mor.México

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