Advertisement

Biotechnology Techniques

, Volume 6, Issue 5, pp 399–404 | Cite as

Characterization of microbial membranes used for the estimation of biochemical oxygen demand with a biosensor

  • E. Galindo
  • J. L. García
  • L. G. Torres
  • R. Quintero
Article

Summary

A bacterial mixed culture was immobilized in Millipore filters to construct microbial-membranes for BOD determination using an oxygen electrode. The biosensor response was best when 0.5 mg cells were immobilized per cm2 of membrane, at 30°C, pH 7 and 0.05 M phosphate buffer. Reproducible microbial-membranes can be constructed and they can be stored for up to 20 days without appreciable loss of their response characteristics.

Keywords

Biochemical Oxygen Demand Millipore Filter Basal Current Current Difference Bacterial Mixed Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. -.
    Galindo, E., Bautista, D., Pimentel, J., Macias, A. Díaz-Nava, P. and Quintero R., (1983) “Application of microbial electrodes to food industry.Prog. in Food Eng. (C. Cantarelli and C. Peri. Ed), Foster-Verlag, Switzerland, p. 409–412.Google Scholar
  2. -.
    Galindo, E. and Quintero, R., (1983), “Electrodo microbiano para la determinación de la DBO”, in Biotecnologia de enzimas, Ed. C. Huitrón, UNAM, México, 364–368.Google Scholar
  3. -.
    Harita, K., Otani, Y., Hikuma, M. and Yasuda, T., (1985), “BOD quick estimating system utilizing microbial electrode”,Instrumental Control. Water, Waste Water Treatment Transport System. Proc IAWPRC Workshop,4: 529–532.Google Scholar
  4. -.
    Hikuma, M., Suzuki, H., Yasuda, T., Karube, I. and Suzuki, S., (1979), “Amperometric estimation of BOD by using living immobilized yeast”,European J. Appl. Microbiol., 8: 289–297.CrossRefGoogle Scholar
  5. -.
    Karube, I., Matsunaga, T., Mitsuda, S. and Suzuki, S., (1977a), “Microbial electrode BOD sensors”,Biotechnol. Bioeng. 19: 1535–1547.CrossRefGoogle Scholar
  6. -.
    Karube, I., Mitsuda, S., Matsunaga, T. and Susuki, S., (1977b). “A rapid method for estimation of BOD by using immobilized microbial cells”,J. Ferment. Technol. 55(3): 243–248.Google Scholar
  7. -.
    Karube, I., Matsunaga, T. and Susuki, S., (1977c), “A new microbial electrode for BOD estimation”,J. solid Phase Biochem. 2(2): 97–104.Google Scholar
  8. -.
    Karube, I., (1990), “Microbial sensor”,J. Biotechnol.,15, 255–256.CrossRefGoogle Scholar
  9. -.
    Kulys, J., Kadziauskiené, K., (1980), “Yeast BOD sensor”,Biotechnol. Bioeng. 22: 221–226.CrossRefGoogle Scholar
  10. -.
    Public Health Association (1971), Standard methods for examination of water and waste water, 474–478, Yearbook Pub., Chicago, Ill.Google Scholar
  11. -.
    Riedel, K., Renneberg, M. and Scheller., (1988), “A fast estimation of biochemical oxygen demand using microbial sensors”,Appl. Microbiol. Biotechnol. 28: 316–318.Google Scholar
  12. -.
    Strand, S. E. and Carlson, D. A., (1984), “Rapid BOD measurement for municipal waste-water samples using a biofilm electrode”,J. Water Pollut Control Fed. 56: 464–467.Google Scholar

Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • E. Galindo
    • 1
  • J. L. García
    • 1
  • L. G. Torres
    • 1
  • R. Quintero
    • 1
  1. 1.Departmento de Bioingeniería, Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavaca, Mor.México

Personalised recommendations