Bulletin of Experimental Biology and Medicine

, Volume 122, Issue 2, pp 832–834 | Cite as

Effects of thyroid hormones on the transcription and structure of the malic enzyme gene in rat liver

  • A. T. Adylova
  • Ya. Kh. Turakulov
Genetics
Received:
  • 22 Downloads

Abstract

Functionally different chromatin fractions have been isolated whose DNAs are used in hybridization with32P-labeled fragments of the malic enzyme gene. In thyroidectomized animals, homologs of the malic enzyme gene are found predominantly in the transcriptionally inactive chromatin fraction. In triiodothyronine-treated rats, the most intense hybridization signals are obtained from the chromatin fraction DNA hypersensitive to micrococcal nuclease and from DNA associated with the nuclear matrix. Thus, induction of the malic enzyme mRNA with triiodothyronine is attended by rearrangements at the higher levels of DNA packing.

Key Words

thyroid hormones malic enzyme gene structural organization transcription 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. T. Adylova and B. A. Atakhanova,Biokhimiya,51, 112–116 (1986).Google Scholar
  2. 2.
    V. V. Bakaev,Itogi Nauki i Tekhn., Ser.Biol. Khimiya [in Russian], Vol. 16, Moscow (1982).Google Scholar
  3. 3.
    A. A. Karavanov and B. N. Afanas'ev,Molekul. Biol.,17, 213–233 (1983).Google Scholar
  4. 4.
    N. E. Kucherenko, B. A. Tsudzevich, Ya. B. Blyum, and Yu. D. Babenok,Biochemical Model of Chromatin Activity Regulation [in Russian], Kiev (1983).Google Scholar
  5. 5.
    S. T. Maniatis, E. Fritch, and J. Sambrook,Methods of Gene Engineering. Molecular Cloning [Russian translation], Moscow (1984).Google Scholar
  6. 6.
    R. Buttyan and C. A. Olsson,Biochem. Biophys. Res. Commun.,138, 1331–1340 (1986).CrossRefGoogle Scholar
  7. 7.
    H. Cedar,Cell,53, 3–4 (1988).PubMedCrossRefGoogle Scholar
  8. 8.
    J. M. Chirgwin, A. E. Przybyla, R. J. MacDonald, and W. J. Rutter,Biochemistry,18, 5294–5300 (1979).PubMedCrossRefGoogle Scholar
  9. 9.
    F. M. Ciejek, M.-J. Tsai, and B. W. O'Malley,Nature,306, 607–609 (1983).PubMedCrossRefGoogle Scholar
  10. 10.
    A. H. Coleoni and D. J. DeGroot,Endocrinology,106, 1103–1104 (1980).PubMedCrossRefGoogle Scholar
  11. 11.
    F. C. Kafatos, C. W. Jomes, and D. Efstratiades,Nucleic Acid Res.,7, 1541–1552 (1979).PubMedGoogle Scholar
  12. 12.
    B. Levy-Wilson,Biochemistry,22, 1245–1250 (1983).PubMedCrossRefGoogle Scholar
  13. 13.
    S. M. Rose and W. T. Garrard,J. Biol. Chem.,259, 8534–8545 (1984).PubMedGoogle Scholar
  14. 14.
    P. H. Thomas,Proc. Natl. Acad. Sci USA,77, 5201–5205 (1980).PubMedCrossRefGoogle Scholar
  15. 15.
    H. Weintraub and C. Groudine,Science,193, 848–856 (1976).PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • A. T. Adylova
    • 1
  • Ya. Kh. Turakulov
    • 1
  1. 1.Department of Gene Expression Regulation, Institute of GeneticsAcademy of Sciences of the Republic of UzbekistanTashkent

Personalised recommendations