Mammalian Genome

, 1:84

Genetic mapping of thet-complex region on mouse chromosome 17 including theHybrid sterility-1 gene

  • Jiři Forejt
  • Vladimir Vincek
  • Jan Klein
  • Hans Lehrach
  • Milada Loudová-Micková
Original Contributions

Abstract

t-haplotypes occupy a region on chromosome (Chr) 17 which slightly overlaps the ends of theT-H-2 interval. The wild-type form of this 14 centi-Morgan (cM) region was mapped in a multilocus backcross (C57BL/10-T×C3H)F1×C57BL/10 using 15 DNA probes on Southern blots of the DNA extracted from 53 animals which were recombinants in theT-H-2 interval. Each recombinant was also progenytested to ascertain itsHybrid sterility-1 (Hst-1) genotype by crossing to PWB/Ph, aMus musculus-derived inbred strain. The limit of resolution of the cross was 0.27 cM. The map distances have been determined for the DNA loci in theT-H-2 interval and theHst-1 gene was mapped in close vicinity to theD17Rp17 locus.

Copyright information

© Springer-Verlag New York Inc 1991

Authors and Affiliations

  • Jiři Forejt
    • 1
  • Vladimir Vincek
    • 2
  • Jan Klein
    • 2
  • Hans Lehrach
    • 3
  • Milada Loudová-Micková
    • 1
  1. 1.Institute of Molecular GeneticsCzechoslovak Academy of SciencesPrague 4Czechoslovakia
  2. 2.Abteilung ImmungenetikMax-Planck-Institut für BiologieTübingenGermany
  3. 3.Imperial Cancer Research Fund LaboratoriesLondonUK

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