Letters in Peptide Science

, Volume 4, Issue 4–6, pp 371–376

Synthesis of an analogue of the lipoglycopeptide membrane intermediate I of peptidoglycan biosynthesis

  • Geneviève Auger
  • Muriel Crouvoisier
  • Martine Caroff
  • Jean van Heijenoort
  • Didier Blanot
Article

DOI: 10.1007/BF02442902

Cite this article as:
Auger, G., Crouvoisier, M., Caroff, M. et al. Lett Pept Sci (1997) 4: 371. doi:10.1007/BF02442902

Summary

α-Dihydroheptaprenyl-pyrophosphoryl-N-acetylmuramoyl-L-Ala-γ-D-Glu-meso-diaminopimeloyl(N-dansyl)-D-Ala-D-Ala (1), an analogue of lipid I of peptidoglycan biosynthesis, was synthesized from natural UDP-N-acetylmuramoyl-pentapeptide in three steps. Compound1 was shown to be a substrate for the MurG transferase fromEscherichia coli, even in the absence of membranes. When membranes were present, dansylated peptidoglycan was also formed.

Keywords

Dansyl group α-Dihydroheptaprenyl phosphate MurG Phosphoroimidazolidate method Plasma desorption mass spectrometry UDP-N-acetylmuramoyl-pentapeptide 

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Geneviève Auger
    • 1
  • Muriel Crouvoisier
    • 1
  • Martine Caroff
    • 2
  • Jean van Heijenoort
    • 1
  • Didier Blanot
    • 1
  1. 1.URA 1131Université de Paris-SudOrsayFrance
  2. 2.URA 1116, CNRSUniversité de Paris-SudOrsayFrance

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