Letters in Peptide Science

, Volume 10, Issue 5–6, pp 621–629 | Cite as

Chitosan-dextran sulfate nanoparticles for delivery of an anti-angiogenesis peptide

Article

Summary

A novel nanoparticle delivery system has been developed by employing the oppositely charged polymers chitosan (CS) and dextran sulfate (DS), and a simple coacervation process. Under the conditions investigated, the weight ratio of the two polymers is identified as a determining factor controlling particle size, surface charge, entrapment efficiency and release characteristics of the nanoparticles produced. Particles of 223 nm mean diameter were produced under optimal conditions with a zeta potential of approximately −32.6 mV. A maximum of 75% anti-angiogenesis peptide entrapment efficiency was achieved with a CS:DS weight ratio of 0.59∶1. The same nanoparticle formulation also showed slow and sustained peptide release over a period of 6 days. In contrast, the formulation containing a lower ratio of CS:DS (0.5∶1) was found to have reduced entrapment efficiency and more rapid peptide release characteristics. The results of this study suggest that physicochemical and release characteristics of the CS-DS nanoparticles can be modulated by changing ratios of two ionic polymers. The novel CS-DS nanoparticles prepared by the coacervation process have potential as a carrier for small peptides.

Key words

chitosan dextran sulphate nanoparticles peptide delivery sustained-release 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Paul, W. and Sharma, C.P., S.T.P. Pharma Sci., 10 (2000) 5.Google Scholar
  2. 2.
    Janes, K.A., Calvo, P. and Alonso, M.J., Adv. Drug Deliv. Rev., 47 (2001) 83.PubMedCrossRefGoogle Scholar
  3. 3.
    Xu, Y. and Du, Y., Int. J. Pharm., 250 (2003) 215.PubMedCrossRefGoogle Scholar
  4. 4.
    Chew, J.L., Wolfowicz, C.B., Mao, H.-Q., Leong, K.W. and Chua, K.Y., Vaccine, 21 (2003) 2720.PubMedCrossRefGoogle Scholar
  5. 5.
    Mao, H.-Q., Roy, K., Troung-Le, V.L., Janes, K.A., Lin, K.Y., Wang, Y., August, J.T. and Leong, K.W., J. Cont. Rel., 70 (2001) 399.CrossRefGoogle Scholar
  6. 6.
    Takeuchi, H., Yamamoto, H., and Kawashima, Y., Adv. Drug Deliv. Rev., 47 (2001) 39.PubMedCrossRefGoogle Scholar
  7. 7.
    Chen, Y., McCulloch, R.K. and Gray, B. N., J. Cont. Rel., 31 (1994) 49.CrossRefGoogle Scholar
  8. 8.
    Janes, K.A., Fresneau, M.P., Marazuela, A., Fabra, A. and Alonso, M.J., J. Cont. Rel., 73 (2001) 255.CrossRefGoogle Scholar
  9. 9.
    Tiyaboonchai, W., Woiszwillo, J. and Middaugh, C.R., Eur. J. Pharm. Sci., 19 (2003) 191.PubMedCrossRefGoogle Scholar
  10. 10.
    Tiyaboonchai, W., Woiszwillo, J., Sims, R.C. and Middaugh, C.R., Int. J. Pharm., 255 (2003) 139.PubMedCrossRefGoogle Scholar
  11. 11.
    Tobio, M., Gref, R., Sanchez, A., Langer, R. and Alonso, M.J., Pharm. Res., 15 (1998) 270.PubMedCrossRefGoogle Scholar
  12. 12.
    Zhang, Q., Shen, Z. and Nagai, T., Int. J. Pharm., 218 (2001) 75.PubMedCrossRefGoogle Scholar
  13. 13.
    Guerrero, D.Q., Allemann, E., Fessi, H. and Doelker, E., Drug Dev. Ind. Pharm., 24 (1988) 1113.CrossRefGoogle Scholar
  14. 14.
    Bae, D.G., Gho, Y.S., Yoon, W.H. and Chae, C.B., J. Biol. Chem., 275 (2000) 13588.PubMedCrossRefGoogle Scholar
  15. 15.
    Calvo, P., Remunan-Lopez, C., Vila-Jato, J.L. and Alonso, M.J., Pharm. Res., 14 (1997) 1431.PubMedCrossRefGoogle Scholar
  16. 16.
    Bantan-Polak, T., Kassai, M. and Grant, K.B., Anal. Biochem., 297 (2001) 128.PubMedCrossRefGoogle Scholar
  17. 17.
    Calvo, P., Remunan-Lopez, C., Vila-Jato, J.L. and Alonso, M.J., J. Appl. Poly. Sci., 63 (1997) 125.CrossRefGoogle Scholar
  18. 18.
    Fernandez-Urrusuno, R., Calvo, P., Remunan-Lopez, C., Vila-Jato, J.L. and Alonso, M.J., Pharm. Res., 6 (1999) 1576.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Yan Chen
    • 1
    • 2
  • Vellore J. Mohanraj
    • 3
  • John E. Parkin
    • 2
  1. 1.Western Australian Biomedical Research Institute, School of PharmacyCurtin UniversityPerth
  2. 2.School of PharmacyCurtin UniversityPerth
  3. 3.Fourrts (India) Laboratories Private LimitedChennaiIndia

Personalised recommendations