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Detection of genetic relationships among fourArtemia species using randomly amplified polymorphic DNA (RAPD)

  • Yi Sun
  • Yi-Cheng Zhong
  • Wen-Qin Song
  • Run-Sheng Zhang
  • Rui-Yang Chen
Article

Abstract

We have applied the technique of random amplification of polymorphic DNA (RAPD) to the analysis of the relationships among four species of brine shrimp:Artemia franciscana, A. urmiana, A. sinica, andA. parthenogenetica. Seventy ten-base synthetic oligonucleotides were used to amplify a total of 458 distinct fragments. DNA polymorphisms were found in all the species examined; the highest percentage of polymorphic bands was found inA. parthenogenetica, with 28.8 per cent. Each species was scored for the presence or absence of every amplification product and the data entered into a binary data matrix. Cluster analysis was then performed to create a dendrogram using UPGMA by the NTSYS program. There are significant differences between bisexual species and parthenogenetic populations.A. parthenogenetica provided 94 specific molecular markers, while bisexual species gave 27 specific molecular markers.A. sinica is a species distinct from the other Old World bisexual species.

Key words

Artemia genetic polymorphism RAPD 

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References

  1. Abatzopoulos, T.J., Zhang, B. and Sorgeloos, P. 1998.Artemia tibetiana: preliminary characterization of a newArtemia species found in Tibet (People's Republic of China). International study onArtemia. LIX. International Journal of Salt Lake Research 7: 41–44.Google Scholar
  2. Abreu-Grobois, F.A. 1987. A review of the genetics ofArtemia. In P. Sorgeloos, D.A. Bengtson, W. Decleir, and E. Jaspers (eds.)Artemia Research and Its Applications, Vol. 1, pp. 61–69. Universa Press, Wetteren, Belgium.Google Scholar
  3. Abreu-Grobois, F.A. and Beardmore, J.A. 1982. Genetic differentiation and speciation in the brine shrimpArtemia. In: C. Barigozzi (eds.) Mechanisms of Speciation, pp. 345–376. Alan R. Liss, New York.Google Scholar
  4. Badaracco, G., Baratelli, L., Ginelli, E., Meneveri, R., Plevani, P., Valsasnini, P. and Barigozzi, C. 1987. Variation in repetitive DNA and heterochromatin in the genusArtemia. Chromosoma 95: 71–75.CrossRefGoogle Scholar
  5. Badaracco, G., Bellorini, M. and Landsberger, N. 1995. Phylogenetic study of bisexualArtemia using random amplified polymorphic DNA. Journal of Molecular Evolution 41: 150–154.PubMedCrossRefGoogle Scholar
  6. Badaracco, G., Tubiello, G., Benfante, R., Cotelli, F., Maiorano, D. and Landsberger, N. 1991. Highly repetitive DNA sequence in parthenogeneticArtemia. Journal of Molecular Evolution 32: 31–36.PubMedCrossRefGoogle Scholar
  7. Barigozzi, C. 1989. GenusArtemia: problems of systematics. In: G. Persoone, P. Sorgeloos, O. Roels and E. Jaspers (eds.) The Brine ShrimpArtemia, Vol. 1, pp. 147–153. Universa Press, Wetteren, Belgium.Google Scholar
  8. Barigozzi, C., Badaracco, G., Plevani, P., Baratelli, L., Profeta, S., Ginelli, E. and Meneveri, R. 1984. Heterochromatin in the genusArtemia. Chromosoma 90: 332–337.CrossRefGoogle Scholar
  9. Bowen, S.T., Davis, M.L., Fenster, S.R. and Lindwall, G.A. 1980. Sibling species ofArtemia. In G. Persoone, P. Sorgeloos, O. Roels and E. Jaspers (eds.) The Brine ShrimpArtemia, Vol. 1, pp. 155–167. Universa Press, Wetteren, Belgium.Google Scholar
  10. Cai, H.J. and Hou, L. 1991. A study on karyotypes ofArtemia from Liaoning province, China. Natural Science Journal of Liaoning Normal University 14(10): 53–59 [in Chinese].Google Scholar
  11. Cai, Y. 1989. A redescription of the brine shrimp (Artemia sinica). The Wasmann Journal of Biology 47(1–2): 105–110.Google Scholar
  12. Cruces, J., Wonenburger, M.L.G., Diaz-Guerra, M., Sebastiàn, J. and Renart, J. 1986. Satellite DNA in the crustaceanArtemia. Gene 44: 341–345.PubMedCrossRefGoogle Scholar
  13. Garcia, D.K. and Benzie, J.A.H. 1995. RAPD markers of potential use in penaeid prawn (Penaeus monodon) breeding programs. Aquaculture 130: 137–144.CrossRefGoogle Scholar
  14. Garcia, D.K., Faggart, M.A., Rhoades, L. and Alcivar-Warren, A.A. 1994. Genetic diversity of culturedPenaeus vannamei shrimp using three molecular genetic techniques. Molecular Marine Biology and Biotechnology 3(5): 270–280.PubMedGoogle Scholar
  15. Günther, R.T. 1890. Crustacea. In: R.T. Günther (ed.) Contributions to the Natural History of Lake Urmia, N. W. Persia and Its Neighborhood. Journal of Linnaeus Society (Zoology) 27: 394–398.Google Scholar
  16. Hou, L., Cai, H.J., and Zou, X.Y. 1993. A study on isozymes of tenArtemia strains from China. Acta Zoology Sinica 39(1): 30–37 [in Chinese].Google Scholar
  17. Hou, L., Cai, H.J., Zou, X.Y. and Yang, G. 1997. Expression of isozyme genes and taxonomic status of bisexualArtemia from China. Acta Zoology Sinica 43(2): 184–191 [in Chinese].Google Scholar
  18. Kellogg, V.A., 1906. A newArtemia and its life conditions. Science 24: 594–596.Google Scholar
  19. Landsberger, N., Cancelli, S., Carettoni, D., Barigozzi, C. and Badaracco, G. 1992. Nucleotide variation and molecular structure of the heterochromatic repetitiveAlu I DNA in the brine shrimpArtemia franciscana. Journal of Molecular Evolution 35: 486–491.PubMedCrossRefGoogle Scholar
  20. Lenz, P. 1980. Ecology of an alkali-adapted variety ofArtemia from Mono lake, California, USA. In: G. Persoone, P. Sorgeloos, D. Roels and E. Jaspers (eds.) The Brine ShrimpArtemia, Vol. 3. Ecology, Culturing, Use in Aquaculture, pp. 79–96. Universa Press, Wetteren, Belgium.Google Scholar
  21. Orozco-Castillo, C., Chalmers, K.J., Waugh, R. and Powel, W. 1994. Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87: 934–940.CrossRefGoogle Scholar
  22. Perez, M.L., Valverde, J.R., Batuecas, B., Amat, F., Marco, R. and Garrese, R. 1994. Speciation in theArtemia genus: Mitochondrial DNA analysis of bisexual and parthenogenetic brine shrimp. Journal of Molecular Evolution 38: 156–168.PubMedCrossRefGoogle Scholar
  23. Piccinelli, M. and Prosdocimi, T. 1968. Descrizione tassonomica delle due speciesArtemia salina L. eArtemia persimilis N.Sp. Inst. Lomb. (Rend. Sci) B, 102: 113–118.Google Scholar
  24. Pilla, E.J.S. and Beardmore, J.A. 1994. Genetic and morphometric differentiation in OLD WORLD bisexual species ofArtemia (the brine shrimp). Heredity 73: 47–56.Google Scholar
  25. Rowland, L.J. and Levi, A. 1994. RAPD-based genetic linkage map of blueberry derived from across between diploid species (Vaccinium darrowi andV. elliottii). Theor. Appl. Genet. 87: 863–868.CrossRefGoogle Scholar
  26. Scheepers, D., Eloy, M.C. and Briquet, M. 1997. Use of RAPD patterns for clone verification and in studying provenance relationships in Norway Spruce (Picea abies). Theor. Appl. Genet. 94: 480–485.CrossRefGoogle Scholar
  27. Sorgeloos, P. 1991. Clarification with regard to the taxonomic status of bisexualArtemia from Asia. Larviculture &Artemia newsletter 19: 45–46.Google Scholar
  28. Triantaphyllidis, G.V., Griel, G.R.T., Abatzopoulos, T.J., Thomas, K.M., Peleman, J., Beardmore, J.A. and Sorgeloos, P. 1997. International study onArtemia. LVII. Morphological and molecular characters suggest conspecificity of all bisexual European and North AfricanArtemia populations. Marine Biology 129: 477–478.CrossRefGoogle Scholar
  29. Welsh, J. and McClelland, M. 1990. Fingerprinting genomes using PCR with arbitrarily primers. Nucleic Acids Research 18: 7213–7218.PubMedGoogle Scholar
  30. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., and Scott, V.T. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Yi Sun
    • 1
  • Yi-Cheng Zhong
    • 1
  • Wen-Qin Song
    • 1
  • Run-Sheng Zhang
    • 1
  • Rui-Yang Chen
    • 1
  1. 1.Department of BiologyNankai UniversityTianjinPR China

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