Glutathione S-transferase and cytochrome P450 genotypes as risk factors for laryngeal carcinoma
- 51 Downloads
While cigarette smoking and alcohol consumption have been linked to laryngeal squamous cell carcinoma (SCC), the role of genetic factors in determining individual susceptibility is unknown. We describe the role of allelism at the glutathione S-transferase GSTM1, GSTM3, GSTT1 and cytochrome P450 CYPIA1, CYP2E1, CYP2D6 loci in determining individual susceptibility to laryngeal SCC. Enzyme genotypes were determined using polymerase chain reaction and restriction enzyme digestion of leukocyte DNA collected from 269 patients with T1–T4 laryngeal carcinomas and 216 controls. While the frequencies of the heterozygote GSTM1 A/B genotype and the homozygote GSTM3 B/B genotype were statistically significantly lower in the patients with tumors than in controls, the frequency of the GSTT1 null genotype was higher in the patients than in controls. The data suggest that allelism at GST loci mediates susceptibility to SCC of the larynx. GSTM1 A/B and GSTM3 B/B appear to be associated with reduced risk, while GSTT1 null may confer increased risk. These findings are compatible with the view that genetic predisposition is important in determining risk for this cancer.
Key wordsSquamous cell carcinoma Larynx Genetic predisposition Glutathione S-transferase GSTM1, GSTM3 and GSTT1 genotypes
- 3.Irish JC (1994) Oncogenes in head and neck cancer: erb-B and ras oncogene activation in laryngeal carcinoma. In: Smee R, Bridger GP (eds) Laryngeal cancer. Proceedings, 2nd World Congress on Laryngeal Cancer, Sydney, 20–24 February 1994. Elsevier, Amsterdam, p 172Google Scholar
- 4.Jahnke V (1995) Bösartige Tumoren des Larynx. In: Naumann HH, Helms J, Herberhold C, Kastenbauer E (eds) Oto-RhinoLaryngologie in Klinik und Praxis, vol 3, Hals. Thieme, Stuttgart, p 388Google Scholar
- 5.Maier H, Gewelke U, Dietz A, Heller WD (1991) Kehlkopfkrebsrisiko bei chronischem Tabak- und/oder Alkoholkonsum. Otorhinolaryngol Nova 1: 271–277Google Scholar
- 7.Strange RC (1993) The glutathione S-transferase GSTM1 locus and cancer susceptibility. In: Tew K, Mannervik B, Mantle TJ, Pickett CB, Hayes JD (eds) Structure and function of glutathione transferases. CRC Press, Boca Raton, p 160Google Scholar