Biotechnology Techniques

, Volume 5, Issue 4, pp 299–302

Formation of spheroids of adult rat hepatocytes on polylysine-coated surfaces and thier albumin production

  • Yasuyuki Sakai
  • Motoyuki Suzuki
Article

Summary

Adult rat hepatocytes in primary culture formed spheroidal aggregates on the surfaces precoated with more than 0.01 mg-polylysine/mL. Epidermal growth factor (EGF) improved the formation and the maintenance of spheroid. The albumin secretion of the spheroids formed was maintained about 2 weeks at a high level.

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Copyright information

© Science & Technology Letters 1991

Authors and Affiliations

  • Yasuyuki Sakai
    • 1
  • Motoyuki Suzuki
    • 1
  1. 1.Institute of Industrial ScienceUniversity of TokyoTokyoJapan

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