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Biotechnology Techniques

, Volume 5, Issue 4, pp 265–268 | Cite as

New method for detecting rhamnolipids excreted byPseudomonas species during growth on mineral agar

  • Inka Siegmund
  • Fritz Wagner
Article

Summary

A new semi-quantitative agar plate test for the detection of extracellular rhamnolipids has been developed. These biological anionic tensides (biosurfactants) form an insoluble ion pair with the cationic tenside cetyltrimethylammonium bromide and the basic dye methylene blue which was included in mineral agar plates. On the light blue agar, productive colonies ofPseudomonas spec. were surrounded by dark blue halos. The test is specific for anionic biosurfactants and can be applied to other glycolipid producing microorganims.

Keywords

Methylene Blue Biosurfactants Mineral Salt Medium Rhamnolipids Rhamnolipid Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Asmer, H.-J.; Lang, S.; Wray, V.; Wagner, F. (1988): J. Am. Oil Chem. Soc., 65; 1460–1466Google Scholar
  2. Betz, J. (1985): Biotech-Forum, 2; 74–80Google Scholar
  3. Fischer, W.K. (1967); in: Liebmann Münchner Beiträge 2nd ed., R. Oldenbourg Verlag, München, Vol. 9; 24–46Google Scholar
  4. Frautz, B.; Lang, S.; Wagner, F. (1984): 3rd European Congress on Biotechnology, München (FRG), Verlag Chemie, Vol. 1; 79–83Google Scholar
  5. Frautz, B.; Lang, S.; Wagner, F. (1986): Biotechnol. Lett., 8; 757–762CrossRefGoogle Scholar
  6. Graber, M.; Morin, A.; Duchiron, F.; Monsan, P.F. (1988): Enzyme Microb. Technol., 10; 198–206CrossRefGoogle Scholar
  7. Mulligan, C.N.; Cooper, D.G.; Neufeld, R.J. (1985): J. Ferment. Technol., 62 (4); 311–314Google Scholar
  8. Syldatk, C.; Lang, S.; Matulovic, U.; Wagner, F. (1985): Z. Naturforsch., 40c; 61–67Google Scholar
  9. Wagner, F. (1987); Fat Sci. Technol., 89; 586–591Google Scholar
  10. Wong, C.-H.; Mazenod, F.P.; Whitesides, G.M. (1983): J. Org. Chem., 48; 3493–3497 (1983)CrossRefGoogle Scholar

Copyright information

© Science & Technology Letters 1991

Authors and Affiliations

  • Inka Siegmund
    • 1
  • Fritz Wagner
    • 1
  1. 1.Institute of Biochemistry and BiotechnologyTechnical University of BraunschweigBraunschweigGermany

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