Biotechnology Techniques

, Volume 5, Issue 4, pp 265–268 | Cite as

New method for detecting rhamnolipids excreted byPseudomonas species during growth on mineral agar

  • Inka Siegmund
  • Fritz Wagner


A new semi-quantitative agar plate test for the detection of extracellular rhamnolipids has been developed. These biological anionic tensides (biosurfactants) form an insoluble ion pair with the cationic tenside cetyltrimethylammonium bromide and the basic dye methylene blue which was included in mineral agar plates. On the light blue agar, productive colonies ofPseudomonas spec. were surrounded by dark blue halos. The test is specific for anionic biosurfactants and can be applied to other glycolipid producing microorganims.


Methylene Blue Biosurfactants Mineral Salt Medium Rhamnolipids Rhamnolipid Production 
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Copyright information

© Science & Technology Letters 1991

Authors and Affiliations

  • Inka Siegmund
    • 1
  • Fritz Wagner
    • 1
  1. 1.Institute of Biochemistry and BiotechnologyTechnical University of BraunschweigBraunschweigGermany

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