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Biotechnology Techniques

, Volume 5, Issue 4, pp 255–258 | Cite as

Measurement of amyloglucosidase usingP-nitrophenyl β-maltoside as substrate

  • Barry V. McCleary
  • Francoise Bouhet
  • Hugues Driguez
Article

Summary

An enzyme-linked assay for the measurement of amyloglucosidase in commercial enzyme mixtures and crude culture filtrates is described. A method for the synthesis of the substrate employed,p-nitrophenyl β-D-maltoside, is also described. The substrate is used in the presence of saturating levels of β-glucosidase. With a range ofAspergillus sp. culture filtrates, an excellent correlation was found for values obtained with this assay and a conventional assay employing maltose as substrate with measurement of released glucose.

Keywords

Maltose Culture Filtrate Maltotetraose Galactopyranosides Crude Culture Filtrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Science & Technology Letters 1991

Authors and Affiliations

  • Barry V. McCleary
    • 1
  • Francoise Bouhet
    • 2
  • Hugues Driguez
    • 2
  1. 1.Megazyme (Austr.) Pty. Ltd.North RocksAustralia
  2. 2.Centre de Recherches sur les Macromolecules Vegetales (CERMAV-CNRS)Grenoble cedexFrance

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