Biotechnology Techniques

, Volume 5, Issue 4, pp 255–258 | Cite as

Measurement of amyloglucosidase usingP-nitrophenyl β-maltoside as substrate

  • Barry V. McCleary
  • Francoise Bouhet
  • Hugues Driguez


An enzyme-linked assay for the measurement of amyloglucosidase in commercial enzyme mixtures and crude culture filtrates is described. A method for the synthesis of the substrate employed,p-nitrophenyl β-D-maltoside, is also described. The substrate is used in the presence of saturating levels of β-glucosidase. With a range ofAspergillus sp. culture filtrates, an excellent correlation was found for values obtained with this assay and a conventional assay employing maltose as substrate with measurement of released glucose.


Maltose Culture Filtrate Maltotetraose Galactopyranosides Crude Culture Filtrate 
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Copyright information

© Science & Technology Letters 1991

Authors and Affiliations

  • Barry V. McCleary
    • 1
  • Francoise Bouhet
    • 2
  • Hugues Driguez
    • 2
  1. 1.Megazyme (Austr.) Pty. Ltd.North RocksAustralia
  2. 2.Centre de Recherches sur les Macromolecules Vegetales (CERMAV-CNRS)Grenoble cedexFrance

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