Human Evolution

, Volume 4, Issue 4, pp 263–270 | Cite as

DNA preservation in Precolumbian remains from the American Southwest

  • N. L. Shearin
  • E. J. King
  • D. H. O’Rourke
Article

Abstract

Naturally desiccated precolumbian remains from the U.S. Southwest were examined for DNA preservation in a variety of tissue types. Histological examination was used to determine if condition of morphological preservation indicated a tissue choice for nucleic acid extraction. It was found that nucleic acids are regularly present in tissue from these samples and neither macroscopic nor microscopic tissue appearance is an indicator of DNA preservation. Extracted DNA from Southwest specimens was small (<500 bp) but demonstrably human. Future analyses of ancient DNA with allele specific oligonucleotide probes should provide answers to specific anthropological questions about Southwestern Amerindian population dynamics, evolution and connections to modern Pueblo peoples.

Key Words

Mummified remains DAPI DnA mtDNA Histology Polymerase Chain reaction 

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References

  1. Anderson S., Bankier A.T., Barrell B.G., de Bruijn, M.H.L., Coulson A.R., Drouin J., Eperon I.C., Nierlich D.P., Roe B.A., Sanger F., Schreier P.H., Smith A.J.H., Staden R. &Young I.G., 1981.Sequence and organization of the human mitochondrial genome. Nature, 290: 457–465.CrossRefGoogle Scholar
  2. Blin N. &Stafford D.W., 1976.A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Research, 3: 2303–2308.Google Scholar
  3. Cann R.L., Stoneking M. &Wilson A.C. 1987.Mitochondrial DNA and human evolution. Nature, 325: 31–36.CrossRefGoogle Scholar
  4. Cordell L.S., 1984.Prehistory of the Southwest. Academic Press: Orlando.Google Scholar
  5. Doran G.H., Dickel D.N., Ballinger W.E. &Agee O.F., 1986.Anatomical, cellular and molecular analysis of 8,000-yr-old human brain tissue from the Windover archaeological site. Nature, 323: 803–806.CrossRefGoogle Scholar
  6. Gill P., Jeffreys A.J. &Werrett D.J., 1985.Forensic application of DNA ‘fingerprints’. Nature, 318: 578–579.CrossRefGoogle Scholar
  7. Glob P.V., 1969.The Bog People: Iron-Age Man Presenved. Ballantine: New York.Google Scholar
  8. Grayson D.K., 1983.The paleontology of Gatecliff Shelter. Small mammals. In (D. H. Thomas, Ed.)The Archaeology of Monitor Valley. 2. Gatecliff Shelter. Anthropological Papers of the American Museum of Natural History. New York: American Museum of Natural History, 59: 99–135.Google Scholar
  9. Higuchi R., von Beroldingen C.H., Sensabaugh G.F. &Erlich H.A., 1988.DNA typing from single bairs. Nature, 332: 543–546.CrossRefGoogle Scholar
  10. Higuchi R.G., Wrischnik L.A., Oakes E., George M., Tong B. &Wilson A.C. 1987.Mitochondrial DNA of the extinct quagga: relatedness and extent of postmortem change. Journal of Molecular Evolution, 25: 283–287.Google Scholar
  11. Higuchi R., Bowman B., Freiberger M., Oliver A.R. &Wilson A.C., 1984.DNA sequences from the quagga, an extinct member of the horse family. Nature, 312: 282–284CrossRefGoogle Scholar
  12. Johnson P.H., Olsen C.B. &Goodman M. 1985.Isolation and characterization of deoxyribonucleic acid from tissue of the woolly mammoth, Mammuthus Primigenius. Comparative Biochemistry and Physiology, 81: 1045–1051.CrossRefGoogle Scholar
  13. Mathien J. &McGuire R.H., 1986.Ripples in The Chichimec Sea: New Considerations of Southwestern-Mesoamerican Interactions. Southern Illinois University Press: Carbondale.Google Scholar
  14. Pääbo S., Gifford J.A. &Wilson A.C., 1988.Mitochondrial DNA sequences from a 7000-year-old brain. Nucleic Acids Research, 16: 9775–9787.Google Scholar
  15. Pääbo S. &Wilson A.C., 1988.Polymerase chain reaction reveals cloning artefacts. Nature, 334: 387–388.CrossRefGoogle Scholar
  16. Pääbo S., 1987.Molecular genetic methods in archaeology. A prospect. Anthropologischer Anzeiger, 45: 9–17Google Scholar
  17. Pääbo S., 1986.Molecular genetic investigations of ancient human remains. Cold Spring Harbor Symposium on Quantitative Biology, LI: 441–446.Google Scholar
  18. Pääbo S., 1985a.Preservation of DNA in ancient Egyptian mummies. Journal of Archaeological Science, 12: 411–417.CrossRefGoogle Scholar
  19. Pääbo S., 1985b.Molecular cloning of ancient Egyptian mummy DNA. Nature 314: 644–645.CrossRefGoogle Scholar
  20. Reed K.C. &Mann D.A. 1985.Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Research, 13: 7207–7221.Google Scholar
  21. Rogers S.O. &Bendich A.J., 1985.Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissue. Plant Molecular Biology, 5: 69–76.CrossRefGoogle Scholar
  22. Russell W.C., Newman C. &Williamson D.H., 1975.A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature, 253: 461–462.CrossRefGoogle Scholar
  23. Sandison A.T. 1955.The histological examination of mummified material. Stain Technology, 30: 277–283.Google Scholar
  24. Saiki R.K., Gelfand D.H., Stoffel D., Scharf S.J., Higuchi R., Horn G.T., Mullis K.B. &Erlich H.A., 1988.Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239: 487–499.Google Scholar
  25. Southern E.M., 1975.Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology, 98: 503–517.Google Scholar
  26. Wahl G.M., Stern M. &Stark G.R. 1979.Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization using dextran sulfate. Proceedings of the National Acadamy of Science, USA, 76: 3683–3687.CrossRefGoogle Scholar
  27. Wallace D.C., Garrison K. &Knowler W.C. 1985.Dramatic founder effects in Amerindian mitochondrial DNAs. American Journal of Physical Anthropology, 68: 149–155CrossRefGoogle Scholar
  28. Wilson A.C., Cann R.L., Carr S.M., George M., Gyllensten U.B., Helm-Bychowski K.M., Higuchi R.G., Palumbi S.R., Prager E.M., Sage R.D. &Stoneking M., 1985.Mitochondrial DNA and two perspectives on evolutionary genetics. Biological Journal of the Linnean Society, 26: 375–400.Google Scholar

Copyright information

© Editrice II Sedicesimo 1989

Authors and Affiliations

  • N. L. Shearin
    • 1
  • E. J. King
    • 2
  • D. H. O’Rourke
    • 1
  1. 1.Department of AnthropologyUniversity of UtahSalt Lake CityUSA
  2. 2.University of UtahSalt Lake CityUSA

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