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AGE

, Volume 17, Issue 3, pp 87–92 | Cite as

Rate of excretion of vitamin C in human urine

  • Gavin King
  • Michael Beins
  • Jennifer Larkin
  • Brett Summers
  • Alfred B. Ordman
Article

Abstract

The dosage of vitamin C necessary to maintain a level in the urine which could be detected using the 2,6-dichlorophenolindophenol assay was determined with undergraduate students. Students taking 250 mg daily did not excrete significant levels of vitamin C in their urine, while excretion increased at doses from 0.5 to 2 g. A 2 g daily dose caused detectable excretion from about 4 until 16 hr later, on both the first and eighth day. A dose of 500 mg taken every 12 hr led to continuously-detectable levels of vitamin C in the urine. The conclusion is that two conditions are necessary to elevate vitamin c excretion continuously: a dose of at least 500 mg and a dose every 12 hr. This is substantially higher than the U.S. recommended daily allowance and more frequent than administration being used in clinical trials.

Keywords

Clinical Trial Undergraduate Student Human Urine Daily Allowance Detectable Excretion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Davies, M.B., Austin, J., and Partridge, D.A.: Vitamin C: Its Chemistry and Biochemistry. Cambridge, Royal Society of Chemistry, 1991, pp. 7–25.Google Scholar
  2. 2.
    Stare, F.J., and Stare, I.M.: Charles Glen King, 1896–1988. J. Nutr., 118:1272–7, 1988.PubMedGoogle Scholar
  3. 3.
    Roig, M.G., Rivera, Z.S., and Kennedy, J.F.: L-ascorbic acid: an overview. Int. J. Food. Sci. Nutr., 44:59–72, 1993.Google Scholar
  4. 4.
    Block, G.: Vitamin C, Cancer and Aging. Age, 16:55–8, 1993.Google Scholar
  5. 5.
    Marwick, C.: Cancer institute takes a look at ascorbic acid. JAMA, 264:1926, 1990.PubMedCrossRefGoogle Scholar
  6. 6.
    Wittes, R.E.: Vitamin C and Cancer. New Engl. J. Med., 312-178–9, 1985.PubMedCrossRefGoogle Scholar
  7. 7.
    Burr, M.L., Bares, C.J., and Godberg, G.: Incidence for premature rupture of membranes in pregnant women with low leukocyte levels of vitamin C. Eur. J. Clin. Nutr., 39c:387–8, 1985.Google Scholar
  8. 8.
    Kimura, H., Yamada, Y., and Morita, Y.: Dietary ascorbic acid depresses plasma and low density lipoprotein lipid peroxidation in genetically scorbutic rats. J. Nutr., 122:1904–9, 1992.PubMedGoogle Scholar
  9. 9.
    Uchida, K., Nomura, U., and Takase, H.: Effect of vitamin C depletion on serum cholesterol and lipoprotein levels in ODS (od/od) rats unable to synthesize ascorbic acid. J. Nutr., 120:1140–7, 1990.PubMedGoogle Scholar
  10. 10.
    Blanchard, J., Conrad, K.A., and Watson, R.R.: Comparison of plasma, mononuclear and polymorphonuclear leukocyte vitamin C levels in young and elderly women during depletion and supplementation. Eur. J. Clin. Nutr., 43: 97–106, 1989.PubMedGoogle Scholar
  11. 11.
    Chavance, M., Herveth, B., and Fournier, C.: Vitamin status, immunity and infections in an elderly population. Eur. J. Clin. Nutr., 43:827–35, 1989.PubMedGoogle Scholar
  12. 12.
    Vallance, S.: Platelets, Leukocytes and buffy layer vitamin C after surgery. Hum. Nutr., 40c:35–41, 1986.Google Scholar
  13. 13.
    Vojdani, A., and Ghoneum, M.: In vivo effect of ascorbic acid enhancement of human natural killer cell activity. Nutr. Res., 13:753–1993.Google Scholar
  14. 14.
    Pryor, W.A.: The formation of free radicals and the consequences of their reactions in vivo. Photochem. Photobiol., 28:787–801, 1978.PubMedGoogle Scholar
  15. 15.
    Harman, D.: The aging process. Proc. Natl. Acad. Sci. USA, 78:7124–7128, 1981.PubMedGoogle Scholar
  16. 16.
    Tappel, A.L: Vitamin E as the biological lipid antioxidant. Vitam. Horm., 20:493–510, 1962.CrossRefGoogle Scholar
  17. 17.
    Niki, E., Saito, T., Kawakami, A., and Kamiya, Y.: Inhibition of oxidation of methyl-linoleate in solution by vitamin E and vitamin C. J. Biol. Chem., 259:4177–4182, 1984.PubMedGoogle Scholar
  18. 18.
    Harman, D.: Free Radical Theory of Aging: Current Status, in Lipofuscin-1987: State of the Art, edited by Zs.-Nagy, I., New York, Elsevier, 1988, pp. 3–21.Google Scholar
  19. 19.
    Gussow, J.D., and Thomas, P.: Nutrition Debate: Sorting Out Some Answers, Menlo Park, CA, Bull Pub., 1986.Google Scholar
  20. 20.
    Pauling, L.: Vitamin C and the Common Cold, New York, Bantam Books, 1970.Google Scholar
  21. 21.
    Griffith, H.W.: Complete Guide to Vitamins, Minerals, and Supplements, Tucson, AZ, Fisher Books, 1988, p. 2.Google Scholar
  22. 22.
    Levine, M.: New concepts in the biology and biochemistry of ascorbic acid. New Engl. J. Med., 314: 892–902, 1986.PubMedCrossRefGoogle Scholar
  23. 23.
    Levine, M., Cantillena, C.C., and Dhariwal, K.R.: In situ kinetics and ascorbic acid requirements. World Rev. Nutr. Diet., 72:114–27, 1993.PubMedGoogle Scholar
  24. 24.
    Olson, J.A., and Hodges, R.E.: The scientific basis of the suggested new RDA values for vitamins A and C. Nutr. Today, 20:14–15, 1985.Google Scholar
  25. 25.
    Blanchard, J., Conrad., K.A., and Garry, P.J.: Effects of age and intake on vitamin C disposition in females. Eur. J. Clin. Nutr., 44:447–460, 1990.PubMedGoogle Scholar
  26. 26.
    Omaye, T.S., Turnbull, J.D., and Sauberlich, H.E.: Selected Methods for the Determination of Ascorbic Acid in Animal Cells, in Methods Enzymol.: Vitamins and Coenzymes, Vol. 62, edited by McCormick, D.B., and Wright, L.D., New York, Academic Press, 1979, pp. 3–11.Google Scholar

Copyright information

© American Aging Association, Inc. 1994

Authors and Affiliations

  • Gavin King
    • 1
  • Michael Beins
    • 1
  • Jennifer Larkin
    • 1
  • Brett Summers
    • 1
  • Alfred B. Ordman
    • 1
  1. 1.Biochemistry ProgramBeloit CollegeBeloit

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