Digestive Diseases and Sciences

, Volume 28, Issue 5, pp 429–439 | Cite as

Production of acute hemorrhagic pancreatitis in the dog using venom of the scorpion,Buthus quinquestriatus

  • Jose L. Pantoja
  • Ian G. Renner
  • Stephan B. Abramson
  • Hugh A. Edmondson
Original Articles


Acute hemorrhagic pancreatitis has been produced in dogs by two separate intraarterial injections (20 and 10 μg/kg) of venom from the scorpionButhus quinquestriatus. Morphological changes related to the development of the disease were detectable by electron and light microscopy at 1 and 3 hr, respectively, following the injection of venom. Six hours following venom injection, widespread areas of hemorrhage and fat necrosis were observed on the surface of the pancreas and adjacent mesenteries. By 24 hr, areas of fat necrosis more than 1 cm in diameter were present on the surface of the pancreas. No free protease was found in pure pancreatic juice collected at 3, 6, 24, and 96 hr after the injection ofButhus quinquestriatus venom. Amylase concentrations in serum increased to a maximum sevenfold above the basal level at 6–8 hr after injection. Since acute hemorrhagic pancreatitis occurred both with and without pancreatic duct cannulation, it is likely that the pathological process is independent of any venom effect on papillary sphincter tone. The morphological characteristics of the experimental disease appear similar to those observed at autopsy in acute hemorrhagic pancreatitis in humans.


Pancreatitis Amylase Pancreatic Duct Pathological Process Pancreatic Juice 
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  1. 1.
    Snodgrass PJ: Diseases of the pancreas.In Harrison's Principles of Internal Medicine, 8th ed. GW Thorn, RD Adams, E Braunwald, KJ Isselbacher, RG Petersdorf (eds). New York: McGraw-Hill, 1977, pp 1633–1645Google Scholar
  2. 2.
    Banks PA: Acute pancreatitis. Gastroenterology 61:382–397, 1971PubMedGoogle Scholar
  3. 3.
    Lawson DW, Daggett WM, Cibetta JM, Corry RJ, Bartlett MK: Surgical treatment of acute necrotizing pancreatitis. Ann Surg 172:605–617, 1970PubMedGoogle Scholar
  4. 4.
    Brandborg LL: Acute pancreatitis.In Gastrointestinal Disease. MH Sleisenger, JS Fordtran (eds). Philadelphia, WB Saunders, 1978, pp 1409–1439Google Scholar
  5. 5.
    Poon-King T: Myocarditis from scorpion stings. Br Med J 1:374–377, 1963PubMedCrossRefGoogle Scholar
  6. 6.
    Bartholomew C, Murphy JJ, McGeeney KF, Fitzgerald O: Pancreatic response toTityus trinitatis venom. West Indian Med J 23:41–43, 1974PubMedGoogle Scholar
  7. 7.
    Renner IG, Pantoja JL, Douglas AP, Abramson SB, Russell FE, Deck K: The effects of intra-pancreaticoduodenal arterial injection of scorpion and rattlesnake venoms on the canine pancreas.Toxicon (in press)Google Scholar
  8. 8.
    Renner IG, Koch M: Electron microscopic changes in scorpion venom in induced acute hemorrhagic pancreatitis in the dog. Gastroenterology 80:1260, 1981 (abstract)Google Scholar
  9. 9.
    Lampel M, Kern HF: Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secretogogue. Virchow Arch A 373:97–117, 1977CrossRefGoogle Scholar
  10. 10.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275, 1951PubMedGoogle Scholar
  11. 11.
    Renner IG, Rinderknecht H, Douglas AP: Pancreatic secretions in acute pancreatitis. Gastroenterology 75:1090–1098, 1978PubMedGoogle Scholar
  12. 12.
    Geokas MC, Rinderknecht H: Free proteolytic enzymes in pancratic juice of patients with acute pancreatitis. Am J Dig Dis 19:591–598, 1974PubMedCrossRefGoogle Scholar
  13. 13.
    Rinderknecht H, Fleming RM: A new, highly sensitive and specific assay for chymotrypsin. Clin Chim Acta 59:139–146, 1975PubMedCrossRefGoogle Scholar
  14. 14.
    Trapnell J: The natural history and management of acute pancreatitis. Clin Gastroenterol 1:147–166, 1972Google Scholar
  15. 15.
    Renner IG, Savage WT III, Pantoja JL, Renner VJ: Death due to acute pancreatitis—a retrospective analysis of 405 autopsy cases. (submitted), Ann. of Int. Med.Google Scholar
  16. 16.
    Rinderknecht H, Renner IG: Intraductal activation of zymogens of chymotrypsin, carboxypeptidase A and elastase in patients with pancratitis. IRCS Med Sci 4:463, 1976Google Scholar
  17. 17.
    Miyasaka K, Rothman SS: Endocrine secretion of α-amylase by the pancreas. Am J Physiol 241:G170-G175, 1981PubMedGoogle Scholar
  18. 18.
    Sarkisova AS, Kovalenko OK, Kurzanov AN, Korot'ko, GF: Internal secretion of enzymes by the pancreas. Prisposobitel'nye Protsessy Organov Peschevareniya Usloviyakh Klin. Esp. KA Zufarov (ed). 1973, pp 66–70Google Scholar
  19. 19.
    Allan BJ, Journut R, White TT: Intraductal activation of pancreatic zymogens behind a carcinoma of the pancreas. Gastroenterology 65:412–418, 1973PubMedGoogle Scholar
  20. 20.
    Allan BJ, White TT: An alternate mechanism for the formation of protein plugs in chronic calcifying pancreatitis. Digestion 11:428–431, 1974PubMedCrossRefGoogle Scholar
  21. 21.
    Renner IG, Rinderknecht H, Valenzuela JE, Douglas AP: Studies of pure pancreatic secretions in chronic alcoholic subjects without pancreatic insufficiency. Scand J Gastroenterol 15:241–244, 1980PubMedCrossRefGoogle Scholar
  22. 22.
    Gallagher S, Hariharan S, Williams JA: Mechanism of scorpion toxin-induced enzyme secretion in rat pancreas. Gastroenterology 80:970–973, 1981PubMedGoogle Scholar

Copyright information

© Digestive Disease Systems, Inc. 1983

Authors and Affiliations

  • Jose L. Pantoja
    • 1
  • Ian G. Renner
    • 1
  • Stephan B. Abramson
    • 1
  • Hugh A. Edmondson
    • 1
  1. 1.Section of Gastroenterology, Department of Medicine, and Department of PathologyUniversity of Southern California School of MedicineLos Angeles

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