The bialaphos biosynthetic genes ofStreptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster
- 575 Downloads
We have isolated and studied the organization ofStreptomyces hygroscopicus genes responsible for the biosynthesis of the antibiotic herbicide bialaphos. Bialaphos production genes were cloned from genomic DNA using a plasmid vector (pIJ702). Three plasmids were isolated which restored productivity toS. hygroscopicus mutants blocked at different steps of the biosynthetic pathway. Subcloning experiments using other nonproducing mutants showed that four additional bialaphos production genes were also contained on these plasmids. A gene conferring resistance to bialaphos, which was independently cloned using the plasmid vector pIJ61, and an antibiotic-sensitive host (S. lividans), was also linked to the production genes. Cosmids were isolated which defined the location of these genes in a 16 kb cluster.
Key wordsStreptomyces Antibiotics Bialaphos Resistance gene Biosynthetic gene cluster
Unable to display preview. Download preview PDF.
- Crameri R, Davies JE (1985) Increased production of aminoglycosides associated with amplified antibiotic resistance genes. J Antibiot 39:128–135Google Scholar
- Distler J, Mansouri K, Piepersberg W (1985) Streptomycin biosynthesis inStreptomyces griseus: II. Adjacent genomic location of biosynthetic genes and one of two streptomycin resistance genes. FEMS Microbiol Lett 30:151–154Google Scholar
- Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H (1985a) Genetic manipulation of streptomyces. A laboratory manual. The John Innes Foundation, Norwich, EnglandGoogle Scholar
- Imai S, Seto H, Sasaki T, Turuoka T, Ogawa H, Satoh A, Inouye S, Niida T, Otake N (1984) Studies on the biosynthesis of bialaphos (SF1293). 4. Production of phosphonic acid derivatives 2-hydroxyethylphosphonic acid, hydroxymethylphosphonic acid and phosphonoformic acid by blocked mutants ofStreptomyces hygroscopicus SF-1293 and their roles in the biosynthesis of bialaphos. J Antibiot 37:1505–1508PubMedGoogle Scholar
- Imai S, Seto H, Sasaki T, Tsuruoka T, Ogawa H, Satoh A, Inouye S, Niida T, Otake N (1985) Studies on the biosynthesis of bialaphos (SF-1293). 6. Production of N-acetyldemethylphosphinothricin and N-acetylbialaphos by blocked mutants ofStreptomyces hygroscopicus SF-1293 and their roles in the biosynthesis of bialaphos. J Antibiot 38:687–690PubMedGoogle Scholar
- Kondo Y, Shomura T, Ogawa Y, Tsuruoka T, Watanabe H, Totukawa K, Suzuki T, Moriyama C, Yoshida J, Inouye S, Niida T (1973) Studies on a new antibiotic SF-1293. 1. Isolation and physico-chemical and biological characterization of SF-1293 substances. Sci Rep Meiji Seika 13:34–41Google Scholar
- Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, USAGoogle Scholar
- Ogawa Y, Tsuruoka T, Inouye S, Niida T (1973) Studies on a new antibiotic SF-1293. Sci Rep Meiji Seika 13:42–48Google Scholar
- Ogawa H, Imai S, Shimizu T, Satoh A (1982) Genetic recombination in AMPBA producingStreptomyces hygroscopicus by protoplast fusion and cell regeneration. Sci Rep Meiji Seika 21:52–58Google Scholar
- Ohnuki T, Imanaka T, Aiba S (1985) Self-cloning inStreptomyces Google Scholar