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Genetica

, 88:59 | Cite as

Ribosomal RNA genes in Scots pine (Pinus sylvestris L.): Chromosomal organization and structure

  • Päivi Karvonen
  • Matti Karjalainen
  • Outi Savolainen
Article

Abstract

The nuclear 18S, 5.8S and 25S rRNA genes exist as thousands of rDNA repeats in the Scots pine genome. The number and location of rDNA loci (nucleolus organizers, NORs) were studied by cytological methods, and a restriction map from the coding region of the Scots pine rDNA repeat was constructed using digoxigenin-labeled flax rDNA as a probe. Based on the maximum number of nucleoli and chromosomal secondary constrictions, Scots pine has at least eight NORs in its haploid genome. The size of the Scots pine rDNA repeat unit is approximately 27 kb, two- or threefold larger than the typical angiosperm rDNA unit, but similar in size to other characterized conifer rDNA repeats. The intergenic spacer region (IGS) of the rDNA repeat unit in Scots pine is longer than 20 kb, and the transcribed spacer regions surrounding the 5.8S gene (ITS1 and ITS2) span a region of 2.9 kb. Restriction analysis revealed that although the coding regions of rDNA repeats are homogeneous, heterogeneity exists in the intergenic spacer region between individuals, as well as among the rDNA repeats within individuals.

Key words

digoxigenin-labeling nucleolus organizers Pinus restriction map rDNA 

References

  1. Bobola, M. S., D. E. Smith & A. S. Klein, 1992. Five major nuclear ribosomal repeats represent a large and variable fraction of the genomic DNA of Picea rubens and P. Mariana. Mol. Biol. Evol. 9: 125–137.PubMedGoogle Scholar
  2. Clegg, M. T., 1989. Molecular diversity in plant populations, pp. 98–115 in Plant Genetics, Breeding and Genetic Resources, edited by Brown, A. H. D., Clegg, M. T., Kahler, A. L. & Weir, B. S. Sinauer Assoc., Sunderland.Google Scholar
  3. Cullis, C. A., G. P. Greissen, S. W. Gorman & R. D. Teasdale, 1988. The 25S, 18S and 5S ribosomal RNA genes from Pinus radiata D. Don., pp. 34–40 in Molecular Genetics of Forest Tree, edited by Cheliak, W. M. & Yapa, A. C. Petawawa National PNFI Information Report PI-X-80, Canada.Google Scholar
  4. Dhillon, S. S., 1987. DNA in tree species, pp. 298–313 in Cell and Tissue Culture in Forestry vol. 1, edited by J. M. Bonga & D. J. Durzan. Martinus Nijhoff Publ.Google Scholar
  5. Dover, G., 1982. Molecular drive: a cohesive mode of species evolution. Nature 299: 111–116.PubMedCrossRefGoogle Scholar
  6. Eckenrode, V. K., J. Arnold & R. B. Meagher, 1985. Comparison of the nucleotide sequence of soybean 18S rRNA with the sequences of other small-subunit rRNAs. J. Mol. Biol. 21: 259–269.Google Scholar
  7. Ellis, T. H. N., D. R. Davies, J. A. Castleton & I. D. Bedford, 1984. The organization and genetics of rDNA length variants in peas. Chromosoma 91: 74–81.CrossRefGoogle Scholar
  8. Goldsbrough, P. B. & C. A. Cullis, 1981. Characterization of the gene for ribosomal RNA in flax. Nucl. Acids Res. 9: 1301–1309.PubMedGoogle Scholar
  9. Howell, W. M. & D. A. Black, 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015.PubMedCrossRefGoogle Scholar
  10. Hubbell, H. R., 1985. Silver staining as an indicator of active ribosomal genes. Stain Technol. 60: 285–294.PubMedGoogle Scholar
  11. Ingle, J., J. N. Timmis & J. Sinclair, 1975. The relationship between satellite DNA, rDNA redundancy, and genome size in plants. Plant Physiol. 55: 496–501.PubMedCrossRefGoogle Scholar
  12. Kessler, C. & V. Manta, 1990. Specificity of restriction endonucleases and DNA modification methyltransferases — a review (edition 3). Gene 92: 1–248.PubMedCrossRefGoogle Scholar
  13. Kumar, P. S., N. C. Subrahmanyam & D. G. Faris, 1987. Nucleolar behavior in pollen mother cells in the pigeonpea. J. Hered. 78: 366–368.Google Scholar
  14. Learn, G. H. & B. A. Schaal, 1987. Population subdivision for ribosomal DNA repeat variants in Clematis fremontii. Evolution 41: 433–438.CrossRefGoogle Scholar
  15. Long, E. O. & I. B. Dawid, 1980. Repeated genes in eukaryotes. Ann. Rev. Biochem. 49: 727–764.PubMedCrossRefGoogle Scholar
  16. MacPherson, P. & W. G. Filion, 1981. Karyotype analysis and the distribution of constitutive heterochromatin in five species of Pinus. J. Heredity 72: 193–198.Google Scholar
  17. Murray, M. G. & W. F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 8: 4321–4325.PubMedGoogle Scholar
  18. Nairn, C. J. & R. J. Ferl, 1988. The complete nucleotide sequence of the small-subunit ribosomal RNA coding region for the cycad Zamia pumila: phylogenetic implications. J. Mol. Evol. 27: 133–141.PubMedCrossRefGoogle Scholar
  19. Natarajan, A. T., K. Ohba & M. Simak, 1961. Karyotype analysis of Pinus sylvestris. Hereditas 47: 379–382.CrossRefGoogle Scholar
  20. Ohta, T. & G. A. Dover, 1984. The cohesive population genetics of molecular drive. Genetics 108: 501–521.PubMedGoogle Scholar
  21. Polans, N. O., N. F. Weeden & W. F. Thompson, 1986. Distribution, inheritance and linkage relationships of ribosomal DNA spacer length variants in pea. Theor. Appl. Gen. 72: 289–295.CrossRefGoogle Scholar
  22. Pruitt, R. E. & E. M. Meyerowitz, 1986. Characterization of the genome of Arabidopsis thaliana. J. Mol. Biol. 187: 169–183.PubMedCrossRefGoogle Scholar
  23. Ricroch, A., E. B. Peffley & R. J. Baker, 1992. Chromosomal location of rDNA in Allium: in situ hybridization using biotin- and fluorescein-labelled probe. Theor. Appl. Genet. 83: 413–418.CrossRefGoogle Scholar
  24. Rogers, S. O. & A. J. Bendich, 1987. Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol. Biol. 9: 509–520.CrossRefGoogle Scholar
  25. Rogers, S. O., S. Honda & A. J. Bendich, 1986. Variation in the ribosomal RNA genes among individuals of Vicia faba. Plant Mol. Biol. 6: 339–345.CrossRefGoogle Scholar
  26. Saghai-Maroof, M. A., K. M. Soliman, R. A. Jorgensen & R. W. Allard, 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci 81: 8014–8018.PubMedGoogle Scholar
  27. Sambrook, J., E. F. Fritsch & T. Maniatis, 1989. Molecular cloning, a laboratory manual (2nd ed.). Cold Spring Harbor Laboratory Press, 1989.Google Scholar
  28. Sax, K. & H. J. Sax, 1933. Chromosome number and morphology in the conifers. J. Arnold Arbor. XIV: 356–375.Google Scholar
  29. Saylor, L. C., 1971. Karyotype analysis of the genus Pinus —subgenus Pinus. Silv. Gen. 21: 155–163.Google Scholar
  30. Saylor, L. C., 1983. Karyotype analysis of the genus Pinus —subgenus Strobus. Silv. Gen. 32: 119–124.Google Scholar
  31. Schaal, B. A. & G. H. Learn Jr., 1988. Ribosomal DNA variation within and among plant populations. Ann. Missouri Bot. Gard. 75: 1207–1216.CrossRefGoogle Scholar
  32. Schaal, B. A., W. J. Leverich & J. Nieto-Sotelo, 1987. Ribosomal DNA variation in the native plant Phlox divaricata. Mol. Biol. Evol. 4: 611–621.Google Scholar
  33. Venkateswarlu, K. & R. Nazar, 1991. A conserved core structure in the 18S–25S rRNA intergenic region from tobacco, Nicotiana rustica. Plant Mol. Biol. 17: 189–194.PubMedCrossRefGoogle Scholar
  34. Wagner, D. B., G. R. Furnier, M. A. Saghai-Maroof, S. M. Williams, B. P. Dancik & R. W. Allard, 1987. Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. Proc. Natl. Acad. Sci. 84: 2097–2100.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Päivi Karvonen
    • 1
  • Matti Karjalainen
    • 1
  • Outi Savolainen
    • 1
  1. 1.Department of GeneticsUniversity of OuluOuluFinland

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