Planta

, Volume 180, Issue 4, pp 555–561

Osmotic induction of fluid-phase endocytosis in onion epidermal cells

  • K. J. Oparka
  • D. A. M. Prior
  • N. Harris
Article

DOI: 10.1007/BF02411454

Cite this article as:
Oparka, K.J., Prior, D.A.M. & Harris, N. Planta (1990) 180: 555. doi:10.1007/BF02411454
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Abstract

A transient plasmolysis/deplasmolysis (plasmolytic cycle) of onion epidermal cells has been shown to induce the formation of fluid-phase endocytic vesicles. Plasmolysis in the presence of the membrane-impermeant fluorescent probes Lucifer Yellow CH (LYCH) and Cascade Blue hydrazide resulted in the uptake of these probes by fluid-phase endocytosis. Following deplasmolysis, many of the dye-containing vesicles left their parietal positions within the cell and underwent vigorous streaming in the cytoplasm. Vesicles were observed to move within transvacuolar strands and their movements were recorded over several hours by video-microscopy. Within 2 h of deplasmolysis several of the larger endocytic vesicles had clustered around the nuclear membrane, apparently lodged in the narrow zone of cytoplams surrounding the nucleus. In further experiments LYCH was endocytically loaded into the cells during the first plasmolytic cycle and Cascade Blue subsequently loaded during a second plasmolytic cycle. This resulted in the introduction of two populations of endocytic vesicles into the cells, each containing a different probe. Both sets of vesicles underwent cytoplasmic streaming. The data are discussed in the light of previous observations of fluid-phase endocytosis in plant cells.

Key words

Allium Apoplast Endocytosis (fluidphase) Epidermis Plasmolysis/deplasmolysis Vesicle (endocytic) 

Abbreviations

LYCH

Lucifer yellow CH

UV

ultraviolet

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • K. J. Oparka
    • 1
  • D. A. M. Prior
    • 1
  • N. Harris
    • 2
  1. 1.Scottish Crop Research InstituteDundeeUK
  2. 2.Department of Biological SciencesUniversity of DurhamDurhamUK

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