Planta

, Volume 180, Issue 4, pp 502–509

Tracheary-element differentiation in suspension-cultured cells ofZinnia requires uptake of extracellular Ca2+

Experiments with calcium-channel blockers and calmodulin inhibitors
  • Alison W. Roberts
  • Candace H. Haigler
Article

Abstract

Tracheary-element (TE) differentiation in suspension cultures ofZinnia elegans L. mesophyll cells was inhibited by blocking calcium uptake in three ways: 1) reducing the [Ca2+] of the culture medium, 2) blocking calcium channels with the non-permeant cation La3+, and 3) blocking calcium channels with permeant dihydropyridine calcium-channel blockers. Calcium-channel blockers were effective when added at any time between 0 and 48 h after culture initiation; after 48h, calcium sequestration and secondary cell-wall deposition began. In contrast, calmodulin antagonists inhibited TE differentiation when added at the beginning of culture, but not when added after 24h. These results indicate that TE differentiation involves at least two calcium-regulated events: one calmodulin-dependent and occurring shortly after exposure to inductive conditions, and the other calmodulin-independent and occurring just prior to secondary cell-wall deposition.

Key words

Calcium in cell differentiation Calcium-channel blockers Calmodulin antagonists Cell culture and differentiation Tracheary element differentiation Zinnia 

Abbreviations

BA

N6-benzylaminopurine

CPZ

chlorpromazine

DIC

differential interference contrast

DMSO

dimethylsulfoxide

IC50

concentration resulting in 50% inhibition

TE

tracheary element

TFP

trifluoperazine

W-5

N-(6-aminohexyl)-1-naphthylenesulfonamide

W-7

N-(6-aminohexyl)-5-chloro-1-naphthylenesulfonamide

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Alison W. Roberts
    • 1
  • Candace H. Haigler
    • 1
  1. 1.Department of Biological SciencesTexas Tech UniversityLubbockUSA

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