Calcified Tissue International

, Volume 34, Issue 1, pp 291–294

Formation of bone by isolated, cultured osteoblasts in millipore diffusion chambers

  • D. J. Simmons
  • G. N. Kent
  • R. L. Jilka
  • D. M. Scott
  • M. Fallon
  • David V. Cohn
Laboratory Investigations

Summary

Osteoblast-like and osteoclast-like cells freed from neonatal calvaria by sequential enzymatic digestion after 6–7 days in culture were placed in diffusion chambers and implanted in the peritoneal cavities of CD-1 mice. About half of the chambers also contained a dead calvarium to test for the need of an “inducer.” After 20 days, 11 of 18 chambers containing the osteoblast-like cells formed large foci of mineralized bone that corresponded to alkaline phosphatase activity throughout the chambers. Moreover, only type I (i.e., bone) collagen was formed. Occasional deposits of bone were found in only 3 of 22 chambers containing the osteoclast-like cells. The presence of dead bone did not affect any of the results. These data confirm the osteoblast-like nature of the isolated cell populations and demonstrate that these cells retain their differentiated function in culture.

Key words

Bone formation Osteoblasts Osteoclasts Bone Induction Alkaline Phosphatase Cell Culture 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • D. J. Simmons
    • 1
  • G. N. Kent
    • 2
  • R. L. Jilka
    • 2
    • 3
  • D. M. Scott
    • 2
  • M. Fallon
    • 4
  • David V. Cohn
    • 2
    • 3
  1. 1.Division of Orthopedic SurgeryWashington UniversitySt. Louis
  2. 2.Research LaboratoriesV.A. Medical CenterKansas City
  3. 3.University of Kansas Medical CenterKansas City
  4. 4.Department of PathologyJewish Hospital of St. LouisSt. LouisUSA

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