Uptake of collagenolytic enzymes by bone cells during isolation from embryonic rat calvaria
Collagenolytic activity extracted from bone cells, isolated from calvaria with a proteolytic enzyme mixture, was analyzed using14C-labeled collagen and a combination of gel electrophoresis and fluorography. The pattern of collagen degradation products obtained did not include 3/4-fragments, typical of vertebrate collagenases, but was essentially identical to the patterns generated by very dilute samples of the proteolytic enzyme mixture used to isolate the bone cells, and purified collagenase fromClostridium histolyticum (E.C. 18.104.22.168.). These and other results strongly suggest that the previously reported collagenolytic activity of bone cells  is exogenous in origin.
Key wordsBacterial collagenase Vertebrate collagenase Sodium dodecyl sulfate polyacrylamide gel electrophoresis Fluorography
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