Calcified Tissue International

, Volume 33, Issue 1, pp 27–34 | Cite as

Silicon: A requirement in bone formation independent of vitamin D1

  • Edith M. Carlisle


Silicon has been reported to be involved in an early stage of bone formation as a result of earlier in vitro and in vivo studies in this laboratory. It is now possible to demonstrate that silicon exerts an effect on bone formation independent of the action of vitamin D. Day-old cockerels were fed Sideficient and Si-supplemented diets with adequate and no dietary vitamin D under trace element controlled conditions. At the end of 4 weeks chicks receiving dietary vitamin D3 (600 IU) exhibited optimal rates of growth whereas the growth rates of chicks receiving D2 (6000 IU) and no vitamin D were markedly depressed. There were no significant differences between growth of Si-deficient and Si-supplemented chicks on the same level of vitamin D. Skull and bone size was proportional to overall chick growth. However, all chicks on Sideficient diets irrespective of the level of dietary vitamin D had gross abnormalities of skull architecture, the overall skull appearance being narrower and shorter. The frontal area was narrower and the dorsal median line at the front parietal junction was depressed with a narrowing both posterior and laterally, stunting parietal and occipital areas. These abnormal areas showed fewer trabeculae and less calcification. Analyses of skull frontal bones for hexosamine, collagen, noncollagenous protein, and bone mineral demonstrated that the major difference was in collagen content, the Si-deficient skulls showing considerably less collagen at each level of vitamin D. These findings demonstrate that silicon has a significant effect on the bone matrix independent of vitamin D, and support the earlier postulate that silicon is involved in an early stage of bone formation.

Key words

Silicon Skull formation Vitamin D Collagen 


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  1. 1.
    Carlisle, E.M.: Silicon localization and calcification in developing bone, Fed. Proc.28:374, 1969Google Scholar
  2. 2.
    Carlisle, E.M.: Silicon: a possible factor in bone calcification, Science167:179–280, 1970Google Scholar
  3. 3.
    Carlisle, E.M.: A relationship between silicon and calcium in bone formation, Fed. Proc.29:565, 1970Google Scholar
  4. 4.
    Carlisle, E.M.: Silicon, an essential element for the chick, Science178:619–621, 1972PubMedGoogle Scholar
  5. 5.
    Schwarz, K., Milne, D.B.: Growth-promoting effects of silicon in rats, Nature239:333–334, 1972CrossRefPubMedGoogle Scholar
  6. 6.
    Carlisle, E.M.:In vivo requirement for silicon in articular cartilage and connective tissue formation in the chick, J. Nutr.106:478–484, 1976PubMedGoogle Scholar
  7. 7.
    Carlisle, E.M.: A silicon requirement for normal skull formation, J. Nutr. (in press)Google Scholar
  8. 8.
    Muller, S.A., Posner A.S., Firschein, H.E.: Effect of vitamin D-deficiency on the crystal chemistry of bone mineral, Proc. Soc. Exp. Biol. Med.121:844–846, 1966PubMedGoogle Scholar
  9. 9.
    Alexander, G.: Mineral element analyses of plants by means of an emission spectrograph. In H. Wallace (ed.): Regulation of the Micronutrient State of Plants by Chelating Agents and Other Factors, pp. 1–5. Edward Bros., Ann Arbor, Michigan, 1971Google Scholar
  10. 10.
    Antonopoulos, C.A.: Separation of glucosamine and galactosamine on the microgram scale and their quantitative determination, Arkin. Kemi.25:243–247, 1975Google Scholar
  11. 11.
    Spare, P.D.: A stable murexide reagent for the estimation of calcium in micro quantities of serum, Clin. Chem.10:726–729, 1964PubMedGoogle Scholar
  12. 12.
    Woessner, J.: The determination of hydroxyproline in tissue and protein samples containing small proportions of this amino acid, Arch. Biochem. Biophys.93:440–447, 1961CrossRefPubMedGoogle Scholar
  13. 13.
    Rosen, H.: A modified ninhydrin colorimetric analysis for amino acids, Arch. Biochem. Biophys.67:10–15, 1957CrossRefPubMedGoogle Scholar
  14. 14.
    Dixon, W.J., Massey, F.J.: Introduction to Statistical Analysis. McGraw-Hill Co., New York, 1969Google Scholar
  15. 15.
    Carlisle, E.M.: Biochemical and morphological changes associated with long bone abnormalities in silicon deficiency. J. Nutr. (in press)Google Scholar
  16. 16.
    Carlisle, E.M., Alpenfels, W.: A requirement for silicon for bone growth in culture. Fed. Proc.37, 1123, 1978 (abstr.)Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Edith M. Carlisle
    • 1
  1. 1.Environmental and Nutritional Sciences, School of Public HealthUniversity of CaliforniaLos AngelesUSA

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