Calcified Tissue International

, Volume 32, Issue 1, pp 195–199

Decreased breaking strength of diabetic rat bone and its improvement by insulin treatment

  • Padmakar K. Dixit
  • Richard A. Ekstrom
Laboratory Investigation

Summary

A simple instrument is described which measures the breaking strength of rat bones. The apparatus yields reproducible results and is suitable for use in measuring the strength of bones from both large and small animals.

Diabetic rat femurs were more fragile and required less force to break in contrast to those from diabetic rats treated with insulin or normal rats. Daily insulin treatment significantly improved the bone cortical thickness and enhanced their capacity to withstand pressure, although these did not reach the level of the normal controls. The amount of force required to break the bone appears to be related to its cortical thickness and mass.

Key words

Bone Diabetes Insulin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Berney, P.W.: Osteoporosis and diabetes mellitus, J. Iowa Med. Sci.42:10–12, 1952Google Scholar
  2. 2.
    Dixit, P.K., Stern, A.M.K.: Effect of insulin on the incorporation of citrate and calcium into the bones of alloxan-diabetic rats, Calcif. Tissue Int.27:227–232, 1979PubMedGoogle Scholar
  3. 3.
    Rowland, L.O., Jr., Harms, R.H., Wilson, H.R., Ross, I.J., Fry, J.L.: Breaking strength of chick bones as an indication of dietary calcium and phosphorus adequacy, Proc. Soc. Exp. Biol. Med.126:399–401, 1967PubMedGoogle Scholar
  4. 4.
    Wolinsky, I., Kenny, A.D.: Mechanical strength and mineral content of bone from rats fed acetazolamide, Calcif. Tissue Res.16:257–260, 1974CrossRefPubMedGoogle Scholar
  5. 5.
    Waite, L.C.: Carbonic anhydrase inhibitors, parathyroid hormone, and calcium metabolism, Endocrinology91:1160–1165, 1967CrossRefGoogle Scholar
  6. 6.
    Waite, L.C., Volkert, W.A., Kenny, A.D.: Inhibition of bone resorption by acetazolamide in the rat, Endocrinology87:1129–1139, 1970PubMedGoogle Scholar
  7. 7.
    Hoffman, W.S.: A rapid photometric method for the determination of glucose in blood and urine, J. Biol. Chem.120:51–55, 1937Google Scholar
  8. 8.
    McKeown, R.M., Lindsay, M.K., Harvey, S.C., Howes, E.L.: The breaking strength of healing fractured fibulae of rats. II. Observations on a standard diet, Arch. Surg.24:458–491, 1932Google Scholar
  9. 9.
    Weir, J.D. de V., Bell, G.H., Chambers, J.W.: The strength and elasticity of bone in rats on a rachitogenic diet, J. Bone Joint Surg.31B:444–451, 1949PubMedGoogle Scholar
  10. 10.
    Weiss, R., Ickowicz, M.: The influence of cortisone on the healing of experimental fractures in rats, Acta Anat. (Basel)59:163–181, 1964Google Scholar
  11. 11.
    Felts, W.J.L.: An apparatus for determination of breaking strength of small rodent bones, Anat. Rec.148:75–79, 1964CrossRefPubMedGoogle Scholar
  12. 12.
    Menczel, J., Makin, M., Robin, G., Jaye, I., Nadr, E.: Prevalence of diabetes mellitus in Jerusalem: its association with presenile osteoporosis, Isr. J. Med Sci.8:918–919, 1972PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Padmakar K. Dixit
    • 1
  • Richard A. Ekstrom
    • 1
  1. 1.Department of AnatomyUniversity of MinnesotaMinneapolisUSA

Personalised recommendations