Calcified Tissue International

, Volume 32, Issue 1, pp 113–122

A quantitative histologic analysis of the growing long bone metaphysis

  • Donald B. Kimmel
  • Webster S. S. Jee
Laboratory Investigation

Summary

The purpose of this work was to analyze the proximal tibial metaphysis of the 170 g rat in a quantitative histologic fashion which would allow some relation to tissue age to be established. Stained 3 µm thick tissue sections were analyzed with the aid of a Merz grid on an eyepiece reticule and a light microscope. Tissue mass and cell distribution were studied in all areas. The rate of change in tissue mass during aging of the metaphysis was calculated. Two regions of the metaphysis were identified. One, corresponding to the primary spongiosa, less than 4.45 days of age, is a region of high turnover of hard tissue and high numbers of osteoblasts and osteoprogenitor cells. The other, corresponding to the secondary spongiosa, is a region of relatively low net tissue turnover and low numbers of osteoblasts and osteoprogenitor cells. Osteoclasts were found relatively more uniformly distributed through the metaphysis than were osteoblasts and osteoprogenitor cells. The rate of bone formation in the primary spongiosa is 50 times that found in the Haversian bone of the rib of 5-year-old humans and about 500 times that found at the cortical-endosteal surface of ribs of 5-year-old humans. It is argued that both cell distribution and tissue distribution in the metaphysis support the concept that osteoblasts and osteoclasts, rather than osteocytes, are responsible for the maturation of the metaphysis. The inhomogeneous distribution of both cells and tissue in the metaphysis has definite meaning for the interpretation of findings concerning the incorporation of radionuclides into the skeleton.

Key words

Rat Bone Metaphysis Quantitative Aging 

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References

  1. 1.
    Schenk, R., Merz, W.A., Fleisch, H.A., Muhlbauer, R.C., Russell, R.G.G.: Effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) and dichloromethylene diphosphonate (Cl2MDP) on the calcification and resorption of cartilage and bone in the tibial epiphysis and metaphysis of rats, Calcif. Tissue Res.11:196–214, 1973PubMedGoogle Scholar
  2. 2.
    Miller, S.C., Jee, W.S.S.: Ethane-1-hydroxy-1,1-diphosphonate (EHDP) effects on growth and modeling of the rat tibia, Calcif. Tissue Res.19:215–231, 1975Google Scholar
  3. 3.
    Young, M.H., Crane, W.A.J.: Effect of hydrocortisone on the utilization of tritiated thymidine for skeletal growth in the rat, Ann. Rheum. Dis.23:163–168, 1964PubMedCrossRefGoogle Scholar
  4. 4.
    Simmons, D.J., Kunin, A.S.: Autoradiographic and biochemical investigations of the effect of cortisone on the bones of the rat, Clin. Orthop. Rel. Res.55:201–215, 1967Google Scholar
  5. 5.
    Simmons, D.J.: Cellular changes in bones of mice as studied with tritiated thymidine and the effects of estrogen, Clin. Orthop. Rel. Res.26:176–191, 1963Google Scholar
  6. 6.
    Whalen, J.P., Krook, L., MacIntyre, I., Nunez, E.: Calcitonin, parathyroidectomy, and modeling of bones in the growing rat, J. Endocrinol.66:207–212, 1976CrossRefGoogle Scholar
  7. 7.
    Kember, N.F.: Tech. Information Division, Brookhaven National Laboratory Report #5541, 1961Google Scholar
  8. 8.
    Landry, M., Fleisch, H.: The influence of immobilization on bone formation as evaluated by osseous incorporation of tetracycline, J. Bone Joint Surg. [Br.]46:764–771, 1964Google Scholar
  9. 9.
    Mehls, O., Ritz, E., Gilli, G., Schmidt-Gayk, H., Krempien, B., Kourist, B., Wesch, H., Prager, P.: Skeletal changes and growth in experimental uremia, Nephron18:288–300, 1977PubMedCrossRefGoogle Scholar
  10. 10.
    Stump, C.W.: The histogenesis of bone, J. Anat.59:136–154, 1925PubMedGoogle Scholar
  11. 11.
    Kember, N.F.: Cell division in endochondral ossification. A study of cell proliferation in rat bones by the method of tritiated thymidine autoradiography, J. Bone Joint Surg. [Br.]42:824–839, 1960Google Scholar
  12. 12.
    Young, R.W.: Cell proliferation and specialization during endochondral osteogenesis in young rats, J. Cell Biol.14:357–370, 1962CrossRefPubMedGoogle Scholar
  13. 13.
    Kimmel, D.B., Jee, W.S.S.: A rapid plastic embedding technique for preparation of three micron thick sections of decalcified hard tissue, Stain Technol.50:83–86, 1975PubMedGoogle Scholar
  14. 14.
    Merz, W.A., Schenk, R.K.: A quantitative histologic study on bone formation in human cancellous bone, Acta Anat. (Basel)76:1–15, 1970Google Scholar
  15. 15.
    Kimmel, D.B., Jee, W.S.S.: Morphometric measurements in tissue bands isometric to an irregular reference line. In P. Meunier (ed.): Bone Histomorphometry, pp. 97–102. Paris, Armour, 1978Google Scholar
  16. 16.
    Sokal, R.R., Rohlf, F.J.: Biometry. San Francisco, Freeman, 1969Google Scholar
  17. 17.
    Whalen, J.P., Winchester, P., Krook, L., Dische, R., Nunez, E.: Mechanisms of bone resorption in human metaphyseal remodeling, Am. J. Roentgenol.112:526–531, 1971Google Scholar
  18. 18.
    Miller, S.C.: Osteoclast cell surface changes during the egg-laying cycle in Japanese quail, J. Cell Biol.75:104–118, 1977CrossRefPubMedGoogle Scholar
  19. 19.
    Parfitt, A.M.: Physiologic and clinical significance of bone histomorphometric data. In R. Recker (ed.): Bone Histomorphometry: Techniques and Interpretation, CRC (in press)Google Scholar
  20. 20.
    Stover, B.J., Atherton, D.R.: Kinetics of the skeletal retention of Pu(IV), Radiat. Res.60:525–535, 1974PubMedGoogle Scholar
  21. 21.
    Wronski, T.J., Smith, J.M., Jee, W.S.S.: Relation of microdistribution and retention of injected Pu-239 to osteosarcoma incidence in beagles, Radiat. Res. (in press)Google Scholar
  22. 22.
    Frost, H.M.: Tetracycline-based histologic analysis of bone remodeling Calcif. Tissue Res.3:211–237, 1969CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Donald B. Kimmel
    • 1
  • Webster S. S. Jee
    • 1
  1. 1.Division of Radiobiology, Department of PharmacologyUniversity of Utah College of MedicineSalt Lake CityUSA

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