Calcified Tissue International

, Volume 36, Issue 1, pp 129–130 | Cite as

Vitamin-D-dependent calcium-binding-protein and parvalbumin occur in bones and teeth

  • M. R. Celio
  • A. W. Norman
  • C. W. Heizmann
Rapid Communications


Vit-D-Dependent calcium binding protein and parvalbumin have both been detected in ameloblasts and calcified cartilage by immunohistochemical techniques. These two Ca2+ binding proteins may play a crucial role in the local accumulation of Ca+2 ions during the process of mineralization.

The mechanisms underlying the deposition of inorganic substances in bone and teeth during physiologic calcification are still the object of intense debate [1,2]. The hypotheses concerning the factors controlling the initiation of mineralization can be subdivided into three large categories: enzymatic (or non-enzymatic) local elevation of phosphate and calcium [3,4,5,6,7], enzymatic removal of inhibitors of calcification [8] and direct nucleation of CaPO4 crystals on collagen fibrils [9].

In support of the first line of thought we report here the simultaneous occurrence of two different very high affinity Ca2+ binding proteins [vitamin-D-dependent CaBP=VD CaBP and parvalbumin=PV] in bones and teeth. During the studied age period and with immunohistochemical methods, we detected the proteins only in calcified cartilage of bones and in ameloblasts of teeth. We propose that VDCaBP and PV help increase the Ca2+ concentration at the calcification front in some regions involved in mineral deposition.


Fibril Collagen Fibril Mineral Deposition Calcium Binding Calcium Binding Protein 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • M. R. Celio
    • 1
  • A. W. Norman
    • 2
  • C. W. Heizmann
    • 3
  1. 1.Anatomisches Institut der UniversitätZürichSwitzerland
  2. 2.Department of BiochemistryUniversity California at RiversideRiversideUSA
  3. 3.Institut für f. Pharmakologie und Biochemie der UniversitätZürichSwitzerland

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