Calcified Tissue International

, Volume 36, Issue 1, pp 77–82 | Cite as

Assessment of anin vivo diffusion chamber method as a quantitative assay for osteogenesis

  • I. Bab
  • B. A. Ashton
  • G. T. Syftestad
  • M. E. Owen
Clinical Investigations


The alkaline phosphatase activity and the calcium and phosphorus content of osteogenic tissue formedin vivo following the implantation of diffusion chambers loaded with rabbit bone marrow cells is reported. (In this study the term osteogenic includes osteoblastic and chondroblastic.) Chambers examined 14–70 days after implantation revealed progressive accumulation of mineral. Alkaline phosphatase activity increased until day 30 and declined thereafter. The osteogenic potential of the marrow cells decreased with increasing weight (age) of the cell donor rabbit when measured either as the percentage of chambers containing osteogenic tissue or as the amount of calcium, phosphorus, or alkaline phosphatase activity within the chambers. The results confirm that measurements of these parameters in tissue formed by cells incubated in diffusion chambersin vivo may be used as a method for assay of osteogenesis.

Key words

Osteogenesis Diffusion chambers Alkaline phosphatase Calcium Phosphorus 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Friedenstein AJ, Chailakhjan RK, Latsinik NV, Panasyuk AF, Keiliss-Borok IV (1974) Stromal cells responsible for transferring the microenvironment of the hemopoietic tissue. Transplantation 17:331–340PubMedGoogle Scholar
  2. 2.
    Budenz RW, Bernard GW (1980) Osteogenesis and leukopoiesis within diffusion chamber implants of isolated bone marrow subpopulations. Am J Anat 159:455–474CrossRefPubMedGoogle Scholar
  3. 3.
    Ashton BA, Allen TD, Howlett CR, Eaglesom CC, Hattori A, Owen ME (1980) Formation of bone and cartilage by marrow stromal cells in diffusion chambersin vivo. Clin Orthopaed Rel Res 151:294–307Google Scholar
  4. 4.
    Ashton Ba, Eaglesom CC, Bab I, Owen ME (in press) Distribution of fibroblastic colony-forming cells in rabbit bone marrow and assay of their osteogenic potential by anin vivo diffusion chamber method. Calcif Tissue IntGoogle Scholar
  5. 5.
    Bourne GH (1972) Phosphatase and calcification. In: Bourne GH (ed) The Biochemistry and physiology of bone, Vol. 2, 2nd ed, Academic Press, New York, London, 79–120Google Scholar
  6. 6.
    Vaughan JM (1970) The physiology of bone. Clarendon Press, OxfordGoogle Scholar
  7. 7.
    Pritchard JJ (1952) A cytological and histochemical study of bone and cartilage formation in the rat. J Anat 86:259–277PubMedGoogle Scholar
  8. 8.
    Reddi AH (1974) Bone matrix in the solid state: geometric influence on differentiation of fibroblasts. Adv Biol Med Phys 15:1–18PubMedGoogle Scholar
  9. 9.
    Eaglesom CC (1980) A study of factors which influence the differentiation of bone tissue. D Phil Thesis, University of OxfordGoogle Scholar
  10. 10.
    Owen M (1982) Bone growth at the cellular level: a perspective. In: Dixon AD, Sarnat BG (eds) Factors and mechanisms influencing bone growth. Alan R. Liss, Inc., New York, 19–28Google Scholar
  11. 11.
    Friedenstein AJ (1980) Stromal mechanisms of bone marrow: cloningin vitro and transformationin vivo. In: Thienfelder S, Rodt H, Kolb HJ (eds) Immunology of bone marrow transplantation. Springer-Verlag, New York, 19–29Google Scholar
  12. 12.
    Tibone KW, Bernard GW (1982) A newin vitro model of intramembranous osteogenesis from adult bone marrow stem cells. In: Dixon AD, Sarnat BG (eds) Factors and mechanisms influencing bone growth. Progress in clinical and biological research, Vol 101. Alan R. Liss, Inc., New York, 107–123Google Scholar
  13. 13.
    Nijweide PJ, van Iperen-van Gent AS, Kawilarang-de Haas EWM, van der Plas A, Wassenaar AM (1982) Bone formation and calcification by isolated osteoblastlike cells. J. Cell Biology 93:318–323CrossRefGoogle Scholar
  14. 14.
    Ashton BA, Owen ME, Eaglesom CC, Parsons JA (1981) Inhibitory action of PTH on the differentiation of osteogenic precursor cells. In: Copp DH, Munson P, Talmage RV (eds) Proceedings VIIth Conference on Calcium Regulating Hormones. Excerpta Medica, 402Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • I. Bab
    • 1
  • B. A. Ashton
    • 1
  • G. T. Syftestad
    • 1
  • M. E. Owen
    • 1
  1. 1.MRC Bone Research LaboratoryNuffield Orthopaedic CentreOxfordUnited Kingdom

Personalised recommendations