Journal of Bone and Mineral Metabolism

, Volume 7, Issue 3, pp 38–43 | Cite as

Effect of ascorbic acid deficiency on calcium metabolism in bone of rat with hereditary defect in L-ascorbic acid biosynthesis

  • Toshitsugu Sugimoto
  • Masaaki Fukase
  • Tohru Tsunenari
  • Masaharu Tsutsumi
  • Susumu Makino
  • Yoshiyuki Hayashi
  • Takuo Fujita
Original Articles
  • 36 Downloads

Abstract

A strain of Wistar rat with a hereditary defect in L-ascorbic acid biosynthesis named osteogenesis disorder (OD) rat was used to explore the effect of ascorbic acid deficiency on bone metabolism. OD rats showed lower levels of serum phosphorus, alkaline phosphatase and urinary hydroxyproline than normal rats. Bone histological studies revealed that the essential feature of OD rats was the failure of bone formation. Very few osteoblasts were seen, but mineralization per se seemed to occur normally despite impaired formation of a new matrix. The cAMP response of the bone to parathyroid hormone (PTH) was examined, using isolated perfused femora. Cyclic AMP response to PTH was significantly lower in OD rats than in normal rats.

OD rats showed a histological picture with severely reduced bone formation and impaired cAMP response to PTH, which suggests that ascorbic acid deficiency might induce osteoblastic insufficiency. OD rats provide us a useful animal model to study the effect of ascorbic acid deficiency on bone metabolism.

Key Words

Ascorbic acid-Bone-Osteoblast 
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Copyright information

© Japanese Society of Bone Metabolism Research 1989

Authors and Affiliations

  • Toshitsugu Sugimoto
    • 1
  • Masaaki Fukase
    • 1
  • Tohru Tsunenari
    • 1
  • Masaharu Tsutsumi
    • 1
  • Susumu Makino
    • 2
  • Yoshiyuki Hayashi
    • 2
  • Takuo Fujita
    • 2
  1. 1.Third Division, Department of MedicineKobe University School of MedicineKobeJapan
  2. 2.Aburabi laboratories, Shionogi Reserch LaboratoriesShionogi & Co.ShigaJapan

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