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Annals of Biomedical Engineering

, Volume 22, Issue 2, pp 218–225 | Cite as

ARTMEM—An interactive graphical program simulating membrane potential measurements across artificial membranes

  • Peter H. Barry
Article

Abstract

This paper describes ARTMEM, an interactive graphical simulation program, and its use for teaching students the concept of membrane potentials and the measurement of electrical potentials across an ion-selective membrane. The program, written in Borland C++, and specifically designed for IBM-PC-compatible equipment, can also run on fast Macintosh computers using SoftPC emulation software. It demonstrates how an ion-selective membrane separating two different salt solutions automaticallygenerates a potential across the membrane, as well as how such electrical potentials can be measured simply and the underlying permeability ratio determined. The program was developed to replace a previous experiment with actual artificial resin membranes; virtually every feature of the experiment has been simulated (e.g., unstirred-layer effects, solutionmixing contamination, liquid junction potential effects). In addition, a number of these features and the procedure for data fitting are more clearly demonstrated using the computer simulation. The efficacy of such a simulation is discussed in comparison with the real experiment and other types of simulations.

Keywords

Physiology teaching Computer simulation Membrane potentials Computer programs 

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References

  1. 1.
    Barry, P.H. Electrical potentials across an artificial membrane. Proc. Aust. Physiol. Pharmacol. Soc. 18:160–166; 1987.Google Scholar
  2. 2.
    Barry, P.H. Membrane potential simulation program for IBM-PC-compatible equipment for physiology and biology students. Proc. Am. J. Physiol. 259 (Adv. Physiol. Educ. 4): S15-S23; 1990.Google Scholar
  3. 3.
    Barry, P.H. From ion channels to membrane potentials: graphical interactive computer exercises designed to emulate laboratory experiments and teach basic principles. Proc. Aust. Physiol. Pharm. Soc., 24:93–103; 1993.Google Scholar
  4. 4.
    Barry, P.H. MEMCABLE: An interactive graphical program simulating passive electrical measure in nerve and muscle cells. Ann. Biomed. Eng. (under revision and review).Google Scholar
  5. 5.
    Barry, P.H.; J.M. Diamond. Function potentials, electrode potentiatis and other problems in interpreting electrical properties of membranes. J. Membrane Biol., 3:93–122; 1970.Google Scholar
  6. 6.
    Barry, P.H.; J.M. Diamond. Effects of unstirred layers on membrane phenomena. Physiol. Rev. 64:763–872; 1984.PubMedGoogle Scholar
  7. 7.
    Barry, P.H.; J.W. Lynch. Topical Review. Liquid junction potentials and small cell effects in patch-clamp analysis. J. Membrane Biol. 121:101–117; 1991.CrossRefGoogle Scholar
  8. 8.
    Goldman, D. Potential, impedance and rectification in membranes. J. Gen Physiol. 27:37–60; 1943.CrossRefGoogle Scholar
  9. 9.
    Hodgkin, A.L.; B. Katz. The effects of sodium ions on the electrical activity of the giant axon of the squid. J. Physiol. (Lond.) 108:37–77; 1949.Google Scholar
  10. 10.
    Macinnes, D.A. The Principles of Electrochemistry, Dover: New York, 1961, pp. 220–245.Google Scholar
  11. 11.
    Manalis, R.S.; L. Hastings. Electrical gradients across an ion-exchange membrane in student's artificial cell. J. Applied Physiol. 36:769–770; 1974.Google Scholar
  12. 12.
    Robinson, R.A.; R.H. Stokes. Electrolyte Solutions (2nd ed., revised). Butterworths; London; 1965, pp. 463–465.Google Scholar

Copyright information

© Biomedical Engineering Society 1994

Authors and Affiliations

  • Peter H. Barry
    • 1
  1. 1.School of Physiology and PharmacologyUniversity of New South WalesKensingtonAustralia

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