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The Histochemical Journal

, Volume 27, Issue 7, pp 547–554 | Cite as

Spatial distribution of L-selectin (CD62L) on human lymphocytes and transfected murine L1-2 cells

  • Sharon R. Hasslen
  • Ulrich H. von Andrian
  • Eugene C. Butcher
  • Robert D. Nelson
  • Stanley L. Erlandsen
Papers

Summary

We have examined the topographical distribution of L-selectin on surface membrane domains of human lymphocytes and murine L1-2 cells transfected to express human L-selectin. L-selectin was immunolocalized using murine monoclonal DREG 200 Fab antibody and a 12 nm colloidal gold-conjugated secondary antibody. Cell surface morphology and surface distribution of gold-labelled L-selectin were visualized using backscatter electron images obtained by high-resolution, field emission scanning electron microscopy. The topographical morphologies of lymphocytes of both types were complex. The surface of human lymphocytes was composed of both microvilli and ruffles; that of the murine cells was composed of long microvilli and few, if any, ruffles. L-selectin on human lymphocytes was observed primarily as focal clusters on the apical surfaces of ruffles and microvilli. Similarly, on the transfected murine cells, L-selectin was detected predominantly on the apical surface of microvilli. We conclude that L-selectin has a common spatial distribution and clustered organization on all leukocytes examined to-date, and that these features of receptor expression likely facilitate rolling of circulating leukocytes on the endothelial surface.

Keywords

Field Emission Scanning Electron Microscopy Surface Membrane Field Emission Scanning Emission Scanning Electron Backscatter Electron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Sharon R. Hasslen
    • 1
  • Ulrich H. von Andrian
    • 2
  • Eugene C. Butcher
    • 3
  • Robert D. Nelson
    • 4
  • Stanley L. Erlandsen
    • 5
  1. 1.Department of Laboratory Medicine and PathologyUniversity of MinnesotaMinneapolisUSA
  2. 2.The Center for Blood Research, Harvard Medical SchoolBostonUSA
  3. 3.Department of PathologyStanford UniversityStanfordUSA
  4. 4.Department of DermatologyUniversity of MinnesotaMinneapolisUSA
  5. 5.Department of Cell Biology and NeuroanatomyUniversity of MinnesotaMinneapolisUSA

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