Journal of tissue culture methods

, Volume 13, Issue 4, pp 275–283

Cryopreservation of embryonic chick myogenic lineage-committed stem cells

  • Henry E. Young
  • Donna C. Morrison
  • Jonathan D. Martin
  • Paul A. Lucas
Contributed Papers

Summary

Undifferentiated embryonic chick stem cells provide a good in vitro test system to examine the effects of recombinant growth factors on the resultant phenotypic expression of these cells. One of the major difficulties in using freshly isolated cells is variation in the proportion of stem cells recovered from different cell harvests. Variation is of particular concern when multiple cell harvests are necessary to provide cells for assaying a single growth factor. As an attempt to minimize this variation, we have examined the efficacy of cryopreserving myogenic lineage-committed stem cells derived from embryonic chick leg muscle. Percent recovery, viability, differentiation morphology, and cellular proliferation rates were compared between sister cultures of freshly isolated and cryopreserved stem cells. Embryonic chick stem cells retained their capacity to differentiate myogenically in culture when slowly frozen in and recovered from 7.5% dimethyl sulfoxide medium supplemented with horse serum and stage-specific embryo extract.

Key words

cryopreservation embryonic chick myogenic stem cells 

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Copyright information

© Tissue Culture Association 1991

Authors and Affiliations

  • Henry E. Young
    • 1
    • 2
  • Donna C. Morrison
    • 1
  • Jonathan D. Martin
    • 1
  • Paul A. Lucas
    • 2
    • 3
  1. 1.Division of Basic Medical SciencesMercer University School of MedicineMacon
  2. 2.Department of SurgeryMercer University School of MedicineUSA
  3. 3.Department of SurgeryMedical Center of Central GeorgiaMacon

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