Antonie van Leeuwenhoek

, Volume 50, Issue 5–6, pp 545–555 | Cite as

The interaction between electron transfer, proton motive force and solute transport in bacteria

  • W. N. Konings
  • K. J. Hellingwerf
  • M. G. L. Elferink


The properties of proton solute symport have been studied inStreptococcus cremoris, Rhodopseudomonas sphaeroides andEscherichia coli. In the homolactic fermentative organismS. cremoris the efflux of lactate is a membrane proteinmediated process, which can lead to the generation of a proton motive force. These observations support the energy-recycling model that postulates the generation of metabolic energy by end-product efflux. Studies with oxidants and reductants and specific dithiol reagents inE. coli membrane vesicles demonstrated the presence of two redox-sensitive dithiol-disulphide groups in the transport proteins of proline and lactose. The redox state of these groups is controlled by the redox potential of the environment and by the proton motive force. One redox-sensitive group is located at the inner surface, the other at the outer surface of the membrane. InRps. sphaeroides andE. coli the activity of several transport proteins depends on the activity of the electron transfer systems.

On the basis of these results a redox model for proton solute transport coupled in parallel to the electron transfer system is postulated.


Lactate Proline Electron Transfer Lactose Metabolic Energy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Kluwer Academic Publishers 1984

Authors and Affiliations

  • W. N. Konings
    • 1
  • K. J. Hellingwerf
    • 1
  • M. G. L. Elferink
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands

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