Plant and Soil

, Volume 98, Issue 3, pp 425–428 | Cite as

Nitrogen-fixing (acetylene-reducing) bacteria associated with ectomycorrhizae of Douglas-fir

  • C. Y. Li
  • L. L. Hung
Short Communications


Nitrogenase activity, measured by acetylene reduction, was detected on nursery-grown, surface-sterilized ectomycorrhizae of Douglas-fir, formed withLaccaria laccata, Hebeloma crustuliniforme, Rhizopogon vinicolor, andThelephora sp. Detached mycorrhizae were incubated in nitrogen-free liquid medium under microaerophilic conditions. Nitrogenase activity was attributed toClostridium spp. andAzospirillum spp.

Key words

Diazotrophs Ectomycorrhizae Nitrogen fixation 


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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • C. Y. Li
    • 1
  • L. L. Hung
    • 2
  1. 1.Forestry Sciences LaboratoryUS Department of Agriculture, Forest Service, Pacific Northwest Research StationCorvallisUSA
  2. 2.Department of Soil ScienceUniversity of FloridaGainesvilleUSA

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