Plant and Soil

, Volume 98, Issue 3, pp 425–428 | Cite as

Nitrogen-fixing (acetylene-reducing) bacteria associated with ectomycorrhizae of Douglas-fir

  • C. Y. Li
  • L. L. Hung
Short Communications

Summary

Nitrogenase activity, measured by acetylene reduction, was detected on nursery-grown, surface-sterilized ectomycorrhizae of Douglas-fir, formed withLaccaria laccata, Hebeloma crustuliniforme, Rhizopogon vinicolor, andThelephora sp. Detached mycorrhizae were incubated in nitrogen-free liquid medium under microaerophilic conditions. Nitrogenase activity was attributed toClostridium spp. andAzospirillum spp.

Key words

Diazotrophs Ectomycorrhizae Nitrogen fixation 

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References

  1. 1.
    Agarwal A K and Keister D L 1983 Appl. Environ. Microbiol. 45, 1592–1601.PubMedGoogle Scholar
  2. 2.
    Barber L E and Evans H J 1975 Can. J. Microbiol. 22, 254–260.Google Scholar
  3. 3.
    Barberaet al. 1985In Nitrogen Fixation Research Progress. Eds. H J Evanset al., p 428. Martinus Nijhoff, Boston.Google Scholar
  4. 4.
    Botheet al. 1985In Nitrogen Fixation Research Progress. Eds. H J Evanset al., p 373. Martinus Nijhoff, Boston.Google Scholar
  5. 5.
    Brouzeset al. 1969 Can. J. Microbiol. 15, 715–723.Google Scholar
  6. 6.
    Döbereiner J 1980In Methods for Evaluating Biological Nitrogen Fixation. Ed. F J Bergersen, pp 535–555. John Wiley and Sons, New York.Google Scholar
  7. 7.
    Emtsev V T 1960 Mikrobiologiya 29, 529–535.Google Scholar
  8. 8.
    Lakshmi V Aet al. Proc. Indian Acad. Sci. 86B, 397–404.Google Scholar
  9. 9.
    Malajczuk N 1979 Aust. J. Bot. 27, 255–272.Google Scholar
  10. 10.
    Markwell M A Ket al. 1978 Analytical Biochem. 87, 206–210.Google Scholar
  11. 11.
    Mishustin E N and Yemstev V T 1976In Nitrogen Fixation by Free-living Microorganisms, IBP 6, Ed. W P P Stewart, pp 29–38. Cambridge.Google Scholar
  12. 12.
    Navko Net al. 1983 Soil Sci. Plant Nutr. 29(3), 261–270.Google Scholar
  13. 13.
    Patriquin D G and J Döbereiner 1978 Can. J. Microbiol. 24, 734–742.PubMedGoogle Scholar
  14. 14.
    Rao A V and B Venkateswarlu 1985 Plant and Soil 88, 153–158.Google Scholar
  15. 15.
    Rice W A and E A Paul 1972 Can J. Microbiol. 18, 715–723.PubMedGoogle Scholar
  16. 16.
    Richards B N and G K Voigt 1964 Nature 201, 310–311.Google Scholar
  17. 17.
    Richards B N 1973 Soil Biol. Biochem. 5, 149–152.CrossRefGoogle Scholar
  18. 18.
    Sarig S Yet al. 1985In Nitrogen Fixation Research Progress. Eds. H J Evanset al., p 707. Martinus Nijhoff, Boston.Google Scholar
  19. 19.
    Vose P B 1983 Can. J. Microbiol. 29, 837–850.Google Scholar
  20. 20.
    Yates M G 1985In Nitrogen Fixation Research Progress Eds. H J Evanset al., pp 449–450. Martinus Nijhoff, Boston.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • C. Y. Li
    • 1
  • L. L. Hung
    • 2
  1. 1.Forestry Sciences LaboratoryUS Department of Agriculture, Forest Service, Pacific Northwest Research StationCorvallisUSA
  2. 2.Department of Soil ScienceUniversity of FloridaGainesvilleUSA

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