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Plant and Soil

, Volume 96, Issue 3, pp 303–316 | Cite as

Salt tolerance of two differently drought-tolerant wheat genotypes during germination and early seedling growth

  • A. Mozafar
  • J. R. Goodin
Article

Summary

Osmotic and specific ion effect are the most frequently mentioned mechanisms by which saline substrates reduce plant growth. However, the relative importance of osmotic and specific ion effect on plant growth seems to vary depending on the drought and/or salt tolerance of the plant under study. We studied the effects of several single salts of Na+ and Ca2+−NaCl, NaNO3, Na2SO4, NaHCO3, Na2CO3, and Ca(NO3)2—on the germination and root and coleoptile growth of two wheat (Triticum aestivum L.) cultivars, TAM W-101 and Sturdy, the former being more drought tolerant than the latter. The concentrations used were: 0, 0.02, 0.04, 0.08, 0.16, and 0.32 mol L−1. Significant two- and three-way interactions were observed between cultivar, kind of salt, and salt concentration for germination, growth of coleoptile and root, and root/coleoptile ratio. Salts differed significantly (P<0.001) in their effect on seed germination, coleoptile and root growth of both cultivars.

Germination of TAM W-101 seeds was consistently more tolerant than that of Sturdy to NaCl, CaCl2, Ca(NO3)2, and NaHCO3 salts at concentrations of 0.02, 0.04, 0.08, 0.16 mol L−1. The osmotic potential, at which the germination of wheat seeds was reduced to 50% of that of the control, was different depending on the kind of salt used in the germination medium. NaCl at low concentrations (0.02 and 0.04 mol L−1) stimulated the germination of both wheat cultivars. At concentrations of 0.02 to 0.16 mol L−1, Ca2+ salts (CaCl2 and Ca(NO3)2) were consistently more inhibitory than the respective Na+ salts (NaCl and NaNO3) for germination of Sturdy. This did not consistently hold true for TAM W-101. Among the Na+ salts, NaCl was the least toxic and NaHCO3 and Na2CO3 were the most toxic for seed germination.

Root and coleoptile (in both wheat cultivars) differed in their response to salts. This differential response of coleoptile and root to each salt resulted in seedlings with a wide range of root/coleoptile ratios. For example, the root/coleoptile ratio of cultivar TAM W-101 changed from 2.09 (in the control) to 3.77, 3.19, 2.8, 2.44, 1.31, 0.32, and 0.0 when subjected to 0.08 mol L−1 of Na2SO4, NaCl, CaCl2, NaNO3, Ca(NO3)2, NaHCO3, and Na2CO3, respectively. Na2CO3 at 0.08 mol L−1 inhibited root growth to such an extent that germinated wheat seeds contained coleoptile but no roots. The data indicate that, apart from the clear and more toxic effects of NaHCO3 and Na2CO3 and lesser toxic effect of NaCl on germination and seedling growth, any toxicity-ranking of other salts done at a given concentration and for a given tissue growth may not hold true for other salt concentrations, other tissues and/or other cultivars.

The more drought-tolerant TAM W-101, when compared to the less drought tolerant Sturdy, showed higher tolerance (at most concentrations) to NaCl, CaCl2, Ca(NO3)2 and NaHCO3 during its seed germination and to Na2SO4 and CaCl2 for its root growth. This supports other reports that some drought-tolerant wheat cultivars are more tolerant to NaCl. In contrast, the coleoptile growth of drought-sensitive Sturdy was noticeably more tolerant to NaNO3, Ca(NO3)2 and NaHCO3 than that of drought-tolerant TAM W-101. Based on the above and the different root/coleoptile ratios observed in the presence of various salts, it is concluded that in these wheat cultivars: a) coleoptile and root tissues are differently sensitive to various salts, and b) at the germination stage, tolerance to certain salts is higher in the more drought-tolerant cultivar.

Key words

Bicarbonate Calcium Carbonate Chloride Coleoptile Germination Nitrate Root Salinity Sodium Sulphate Triticum aestivum Wheat 

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References

  1. 1.
    Ahmed S U 1985 Germination and seedling growth characteristics of some cultivated and wild selections of wheat cultured in sea water. J. Arid Environ. 8, 133–139.Google Scholar
  2. 2.
    Anderson W P and Collins J C 1969 The exudate from excised maize roots bathed in sulphate media. J. Exp. Bot. 20, 72–80.Google Scholar
  3. 3.
    Ashraf C M and Abu-Shakra S 1978 Wheat seed germination under low temperature and moisture stress. Agron. J. 70, 135–139.Google Scholar
  4. 4.
    Ayers A D and Hayward H E 1948 A method for measuring the effects of soil salinity on seed germination with observations on several crop plants. Soil Sci. Soc. Amer. Proc. 13, 224–226.Google Scholar
  5. 5.
    Ben-Zioni A, Vaadia Y and Lips S H 1970 Correlation between nitrate reductase, protein synthesis and malate accumulation. Physiol. Plant. 23, 1039–1047.Google Scholar
  6. 6.
    Bernstein L 1964 Effects of salinity on mineral composition and growth of plants. Plant Anal. Fert. Problems Colloq. 4, 25–45.Google Scholar
  7. 7.
    Bernstein L 1975 Effects of salinity and sodicity on plant growth. Annu. Rev. Phytopathol. 13, 295–312.CrossRefGoogle Scholar
  8. 8.
    Bernstein L and Hayward H E 1958 Physiology of salt tolerance. Annu. Rev. Plant Physiol. 9, 25–46.CrossRefGoogle Scholar
  9. 9.
    Bohm W 1979 Methods of Studying Root Systems. Springer-Verlag, New York.Google Scholar
  10. 10.
    Brown J W, Wadleigh C H and Hayward H E 1953 Foliar analysis of stone fruit and almond trees on saline substrates. Proc. Am. Soc. Hort. Sci. 61, 49–55.Google Scholar
  11. 11.
    Cram W J 1974 Effect of Cl on HCO3 and malate fluxes and CO2 fixation in carrot and barley root cells. J. Exp. Bot. 25, 253–268.Google Scholar
  12. 12.
    Dijkshoorn W, Lathwell D J and Wit C J De 1968 Temporal changes in carboxylate content of rye grass with stepwise change in nutrition. Plant and Soil 29, 369–389.CrossRefGoogle Scholar
  13. 13.
    Frost W B, Blevins D B and Banett M N 1978 Cation pretreatment effects on nitrate uptake, xylem exudate, and malate levels in wheat seedlings. Plant Physiol. 61, 323–326.Google Scholar
  14. 14.
    Hayward H E and Wadleigh C H 1949 Plant growth on saline and alkali soils. Adv. Agron. 1, 1–35.Google Scholar
  15. 15.
    Hendricks S B 1974 Promotion of seed germination by nitrate, nitrite, hydroxylamine and ammonium salts. Plant Physiol. 54, 304–309.Google Scholar
  16. 16.
    Hepler P K and Wayne R O 1985 Calcium and plant development. Annu. Rev. Plant Physiol. 36, 397–439.CrossRefGoogle Scholar
  17. 17.
    Hurd R G 1958 The effect of pH and bicarbonate ions on the uptake of salts by disks of red beet. J. Exp. Bot. 9, 159–174.Google Scholar
  18. 18.
    Hyder S Z and Yasmin S 1972 Salt tolerance and cation interaction in alkali sacaton at germination. J. Range Manage. 25, 390–392.Google Scholar
  19. 19.
    Jackson W A and Coleman N T 1959 Ion absorption by bean roots and organic acid changes brought about through CO2 fixation. Soil Sci. 87, 311–319.Google Scholar
  20. 20.
    Jacobson L and Ordin L 1954 Organic acid metabolism and ion absorption in roots. Plant Physiol. 29, 70–75.Google Scholar
  21. 21.
    Jacoby B and Laties G G 1971 Bicarbonate fixation and malate compartmentation in relation to salt-induced stoichiometric synthesis of organic acid. Plant Physiol. 47, 525–531.Google Scholar
  22. 22.
    Kirkby E A and Pilbeam D J 1984 Calcium as a plant nutrient. Plant Cell Environ. 7, 397–405.Google Scholar
  23. 23.
    Kirkham M B 1984 Water relations of drought-resistant and drought-sensitive wheat cultivars sprinkled with saline water. Irrig. Sci. 5, 137–146.CrossRefGoogle Scholar
  24. 24.
    Kirkham M B, Smith E L, Dhanasobhon C and Drake T I 1980 Resistance to water loss of winter wheat flag leaves. Cereal Res. Commun. 8, 393–398.Google Scholar
  25. 25.
    Lee J A and Woolhouse H W 1969 A comparative study of bicarbonate inhibition of root growth in calciole and calcifuge grasses. New Phytol. 68, 247–255.Google Scholar
  26. 26.
    Levitt J 1972 Responses of Plants to Environmental Stresses. Academic Press, New York. Pp. 489–530.Google Scholar
  27. 27.
    Mengel K, Bubl W and Scherer H W 1984 Iron distribution in vine leaves with HCO3−1 induced chlorosis. J. Plant Nutr. 7, 715–724.Google Scholar
  28. 28.
    Miller G W and Evans H J 1956 Inhibition of plant cytochrome oxidase by bicarbonate. Nature 178, 974–976.Google Scholar
  29. 29.
    Mozafar A and Goodin J R 1986 Effect of contact site and water supply on germination and seedling growth of wheat. Seed Sci. Tech. 14, 245–251.Google Scholar
  30. 30.
    Overstreet R, Ruben A and Broyer T C 1940 The absorption of bicarbonate ions by barley plants as indicated by studies with radioactive carbon. Proc. Natl. Acad. Sci. U.S. 26, 688–695.Google Scholar
  31. 31.
    Paliwal K V and Gandhi A P 1968 Anion effect of germination of some jowar and paddy varieties in saline substrate. Indian J. Plant Physiol. 11, 62–67.Google Scholar
  32. 32.
    Poljakoff-Mayber A and Gale J 1975 Plants in Saline Environments. Springer-Verlag. New York.Google Scholar
  33. 33.
    Porter L K and Thorne D W 1955 Interrelation of carbon dioxide and bicarbonate ions in causing plant chlorosis. Soil Sci. 79, 373–382.Google Scholar
  34. 34.
    Puntamkar S S, Sharma D C, Sharma O P and Seth S P 1970 Effect of common salts of sodium and calcium on the germination of different wheat varieties (Triticum aestivum L.) Indian J. Plant Physiol. 13, 233–239.Google Scholar
  35. 35.
    Redmann R E 1974 Osmotic and specific ion effects on the germination of alfalfa. Can. J. Bot., 52, 803–808.Google Scholar
  36. 36.
    Roundy B A, Young J A and Evans R A 1985 Germination of basin wildrye and tall wheatgrass in relation to osmotic and matric potential. Agron. J. 77, 129–135.Google Scholar
  37. 37.
    Ryan J, Miyamoto S and Stroehlen J L 1975 Salt and specific ion effects on germination of four grass. J. Range Manage. 28, 61–64.Google Scholar
  38. 38.
    Seropian C and Planchon C 1984 Physiological responses of six bread wheat and durum wheat genotypes to water stress. Euphytica 33, 757–767.CrossRefGoogle Scholar
  39. 39.
    Sharma O P, Puntamkar S S and Seth S P 1970 Salt tolerance of different varieties of wheat (Triticum aestivum L.) at germination. Indian. J. Agric. Sci. 40, 929–932.Google Scholar
  40. 40.
    Sharma S K, Joshi Y C and Bal A R 1984 Osmotic and ionic effects in salt sensitive and resistant wheat varieties. Indian J. Plant Physiol. 27, 153–158.Google Scholar
  41. 41.
    Smith A F and Raven J A 1979 Intracellular pH and its regulation. Annu. Rev. Plant Physiol. 30, 289–311.CrossRefGoogle Scholar
  42. 42.
    Strogonov B P 1970 Structure and Function of Plant Cells in Saline Habitats. New Trends in the Study of Salt Tolerance. Israel Prog. Sci. Transl., Jerusalem, John Wiley, New York.Google Scholar
  43. 43.
    Ungar I A 1974 The effect of salinity and temperature on seed germination and growth ofHordeum jubatum. Can. J. Bot. 52, 1357–1362.Google Scholar
  44. 44.
    Ungar I A 1978 Halophyte seed germination. Bot. Rev. 44, 233–264.Google Scholar
  45. 45.
    Vora A B and Patel M K 1975 Promotive effect of NaCl and CaCl2 mixtures and CaCl2 and MgCl2 mixtures on extension growth of guar seedlings. Curr. Sci. 44, 783–784.Google Scholar
  46. 46.
    Waisel Y 1985 The stimulating effects of NaCl on root growth of Rhodes grass (Chloris gayana). Physiol. Plant. 64, 519–522.Google Scholar
  47. 47.
    Wallace A, Abou-Zamzam A M and Motoyama E 1971 Cation and anion balance in the xylem exudate of tobacco roots. Plant and Soil 35, 433–438.Google Scholar
  48. 48.
    Yadava R B R, Mehra, K L, Magoon M L, Sreenath P R and Yadav M S 1975 Varietal differences in salt tolerance during seed germination of guar. Indian J. Plant Physiol. 18, 16–19.Google Scholar
  49. 49.
    Younis A F and Hatata M A 1971 Studies on the effects of certain salts on germination, on growth of root, and on metabolism. I. Effect of chlorides and sulphates of sodium, potassium and magnesium on germination of wheat grains. Plant and Soil 34, 183–200.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • A. Mozafar
    • 1
  • J. R. Goodin
    • 1
  1. 1.Department of BiologyTexas Tech UniversityLubbockUSA

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