Plant and Soil

, Volume 62, Issue 3, pp 413–430 | Cite as

Experimental determination of nitrogen kinetic isotope fractionation: Some principles; illustration for the denitrification and nitrification processes

  • A. Mariotti
  • J. C. Germon
  • P. Hubert
  • P. Kaiser
  • R. Letolle
  • A. Tardieux
  • P. Tardieux


A few principles relative to the presentation and use of nitrogen stable isotopic data are briefly reviewed. Some classical relationships between the isotope composition of a substrate undergoing a single-step unidirectional reaction, are introduced.

They are illustrated through controlled experiments on denitrification in a soil, and through nitrification by pure cultures ofNitrosomonas europaea. In the latter case, the isotope fractionation is calculated from the isotopic composition of the residual substrate, then of the product and the result is shown to be statistically the same for the two procedures.

The isotopic enrichment factor for denitrification is −29.4±2.4‰ at 20°C, and −24.6±0.9‰ at 30°C; for nitrification this factor is −34.7±2.5‰ under the experimental conditions employed.

Key Words

Denitrification Nitrification Nitrogen isotope fractionation Nitrogen-15 natural abundance 


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Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1981

Authors and Affiliations

  • A. Mariotti
    • 1
  • J. C. Germon
    • 2
  • P. Hubert
    • 3
  • P. Kaiser
    • 4
  • R. Letolle
    • 1
  • A. Tardieux
    • 4
  • P. Tardieux
    • 4
  1. 1.Laboratoire de Géologie dynamique, T. 26, 4EUniversité Pierre et Marie CurieParis
  2. 2.Laboratoire de Microbiologie des SolsI.N.R.A.Dijon cedex
  3. 3.Centre d’Informatique GéologiqueE.N.S.M.Fontainebleau
  4. 4.Institut PasteurChaire de Microbiologie, I.N.A.Paris

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