Molecular and Cellular Biochemistry

, Volume 67, Issue 2, pp 161–170 | Cite as

A series of repetitive DNA sequences are associated with human core and H1 histone genes

  • D. Collart
  • G. S. Stein
  • J. L. Stein
Original Articles


Repetitive DNA sequences, derived from the human β-globin gene cluster, were mapped within a series of human genomic DNA segments containing core (H2A, H2B, H3 and H4) and H1 histone genes. Cloned recombinant λCH4A phage with human histone gene inserts were analyzed by Southern blot analysis using the following32P-labeled (nick translated) repetitive sequences as probes:Alu I,Kpn I and LTR-like. A cloned DNA designated RS002-5′C6 containing (i)a (TG)16 simple repeat, (ii) an (ATTTT)n repeat and (iii)a 52 base pair alternating purine and pyrimidine sequence was also used as a radiolabelled hybridization probe. Analysis of 12 recombinant phage, containing 6 arrangements of core histone genes, indicated the presence ofAlu I,Kpn and RS002-5′C6 repetitive sequences. In contrast, analysis of 4 human genomic DNA segments, containing both core and H1 histone genes, indicated the presence of onlyAlu I family sequences. LTR-like sequences were not detected in association with any of the core or H1 histone genes examined. These results suggest that human histone and β-globin genes share certain aspects of sequence organization in flanking regions despite marked differences in their overall structure and pattern of expression.


histone genes globin gene cluster repetitive sequences sequence organization 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Schmid CW, Deininger PL: Cell 6:345–358, 1975.CrossRefPubMedGoogle Scholar
  2. 2.
    Houck CM, Rinehart FP, Schmid CW: J Mol Biol 132:289–306, 1979.CrossRefPubMedGoogle Scholar
  3. 3.
    Lewin B: Gene Expression 2, Second Edition, Eukaryotic Chromosomes, Wiley, New York, 1980.Google Scholar
  4. 4.
    Pearson WR, Wu JR, Bonner J: Biochem 17:51–59, 1978.CrossRefGoogle Scholar
  5. 5.
    Wilkes MM, Pearson WR, Wu JR, Bonner J: Biochem 17:60–69, 1978.CrossRefGoogle Scholar
  6. 6.
    Schmid CW, Jelinek WR: Science 216:1065–1070, 1982.PubMedGoogle Scholar
  7. 7.
    Jagadeeswaran P, Forget BG, Weissman SM: Cell 26:141–142, 1981.CrossRefPubMedGoogle Scholar
  8. 8.
    Calabretta B, Robberson DL, Barrera-Saldana HA, Lambrou TP, Saunders GF: Nature 296:219–225, 1982.CrossRefPubMedGoogle Scholar
  9. 9.
    Rinehart PF, Ritch GT, Deininger LP, Schmid WC: Biochem 20:3003–3010, 1980.CrossRefGoogle Scholar
  10. 10.
    Miyake T, Migita K, Sakaki Y: Nucl Acids Res 11:6837–6846, 1983.PubMedGoogle Scholar
  11. 11.
    Adams JM, Kaufman RE, Kretschmer PJ, Harrison M, Nienhuis AW: Nucl Acids Res 8:6113–6128, 1980.PubMedGoogle Scholar
  12. 12.
    Shafit-Zugardo B, Malo JJ, Brown FL: Nucl Acids Res 10:3175–3193, 1982.Google Scholar
  13. 13.
    Sun L, Paulson KE, Schmid CW, Kadyk L, Leinwand L: Nucl Acids Res 12:2669–2690, 1984.PubMedGoogle Scholar
  14. 14.
    Varmus HE: Science 216:812–820, 1982.PubMedGoogle Scholar
  15. 15.
    Collins FS, Weisman SM: The Molecular Genetics of Human Hemoglobin, Progress in Nucleic Acid Research and Molecular Biology, Academic Press, inc. New York, 1984.Google Scholar
  16. 16.
    Sierra F, Lichtler A, Marashi F, Rickles R, Van Dyke T, Clark S, Wells J, Stein G, Stein J: Proc Natl Acad Sci USA 79:1795–1799, 1982.PubMedGoogle Scholar
  17. 17.
    Gross K, Schaffner W, Telford J, Birnstiel M: Cell 8:479–484, 1976.CrossRefPubMedGoogle Scholar
  18. 18.
    Wu M, Holmes DS, Davidson N, Cohn RH, Kedes LH: Cell 9:163–169, 1976.CrossRefPubMedGoogle Scholar
  19. 19.
    Lifton RP, Goldberg ML, Karp RW, Hogness DS: Cold Spring Harbor Symp Quant Biol 42:1047–1051, 1977.Google Scholar
  20. 20.
    Kedes LH: Ann Rev Bochem 48:837–870, 1979.CrossRefGoogle Scholar
  21. 21.
    Marashi F, Baumbach L, Rickles R, Sierra F, Stein JL, Stien GS: Science 215:683–685, 1982.PubMedGoogle Scholar
  22. 22.
    Sierra F, Leza A, Marashi F, Plumb M, Rickles R, Van Dyke T, Clark S, Wells J, Stein GS, Stein JL: Biochem Biophys Res Comm 104:785–792, 1982.PubMedCrossRefGoogle Scholar
  23. 23.
    Birnboim HC, Doly J: Nucl Acids Res 7:1513–1523, 1979.PubMedGoogle Scholar
  24. 24.
    Ish-Horowicz DD, Burke JF: Nucl Acids Res 9:2989–2998, 1981.PubMedGoogle Scholar
  25. 25.
    Carozzi M, Marashi F, Plumb M, Zimmerman S, Zimmerman A, Wells JRE, Stein G, Stein J: Science 224:1115–1117, 1984.PubMedGoogle Scholar
  26. 26.
    Zwollo P, Stein GS, Stein JL: Biochem Biophys Res Comm 124:988–993, 1984.CrossRefPubMedGoogle Scholar
  27. 27.
    Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982.Google Scholar
  28. 28.
    Maniatis T, Jeffrey A, Kleid DG: Proc Natl Acad Sci USA 72:1184–1188, 1975.PubMedGoogle Scholar
  29. 29.
    Southern EM: J Mol Biol 98:503–517, 1975.PubMedGoogle Scholar
  30. 30.
    Strayer D, Heintz N, Roeder R, Gillespie D: Proc Natl Acad Sci USA 80:4770–4774, 1983.PubMedGoogle Scholar
  31. 31.
    Jelinek R, Toomey TP, Leinwand L, Duncan CH, Biro PA, Choudary PV, Weissman SM, Rubin CM, Houck CM, Deininger PL, Schmid CM: Proc Natl Acad Sci USA 77:1398–1402, 1980.PubMedGoogle Scholar
  32. 32.
    Ariga H: Mol Cell Biol 4:1476–1482, 1984.PubMedGoogle Scholar
  33. 33.
    Grimald G, Singer MF: Nucl Acids Res 11:321–338, 1983.Google Scholar
  34. 34.
    Manley JL, Colozzo MT: Nature 300:376–379, 1982.CrossRefPubMedGoogle Scholar
  35. 35.
    Hess JF, Schmid CW, Shen C-KJ: Science 226:67–70, 1984.PubMedGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • D. Collart
    • 1
  • G. S. Stein
    • 1
  • J. L. Stein
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Department of Immunology and Medical MicrobiologyUniversity of FloridaGainesvilleUSA

Personalised recommendations