Annals of Biomedical Engineering

, Volume 21, Issue 1, pp 77–83 | Cite as

Design and construction of a linear shear stress flow chamber

  • Shunichi Usami
  • Hsuan-Hsu Chen
  • Yihua Zhao
  • Shu Chien
  • Richard Skalak
Article

Abstract

A new paralle plate flow chamber that has a linear variation of shear stress, starting from a predetermined maximum value at the entrance and falling to zero at the exit, has been designed and tested. This is in contrast to the usual rect-angular channel plan which produces a constant shear stress over the entire length. The new design is based on the theory of Hele-Shaw flow between parallel plates. To verify the efficacy of the flow channel, the effect of fluid shear stress on platelet adhesion to a fibrinogen-coated glass surface was tested. The percentage of attached platelets after 5 min of shear stress is shown to be a function of shear stress. With this new flow chamber, cell-cell interactions can be studied efficiently over a wide range of shear stress using a single run at constant discharge.

Keywords

Flow chamber Hele-Shaw flow Cell adhesion 

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Copyright information

© Pergamon Press Ltd. 1993

Authors and Affiliations

  • Shunichi Usami
    • 1
  • Hsuan-Hsu Chen
    • 1
  • Yihua Zhao
    • 2
  • Shu Chien
    • 2
  • Richard Skalak
    • 2
  1. 1.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan
  2. 2.Department of Applied Mechanics and Engineering Sciences/BioengineeringUniversity of CaliforniaLa Jolla

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