Advertisement

Potato Research

, Volume 31, Issue 2, pp 241–246 | Cite as

Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato

  • J. H. M. Hovenkamp-Hermelink
  • J. N. De Vries
  • P. Adamse
  • E. Jacobsen
  • B. Witholt
  • W. J. Feenstra
Article

Summary

Small amounts of potato tuber and leaf tissues are extracted in perchloric acid. After staining with I2-KI solution absorbancies at 618 and 550 nm are measured. The amylose/amylopectin ratio can be estimated from the ratio of the absorbancies by using a formula or a graph in which the specific absorptions of the two compounds are introduced. Weighing of samples is not required. The method can be used for the estimation of the starch composition of minitubers and is especially suitable for the detection of mutants with an altered starch composition.

Additional keywords

minitubers starch composition mutant detection 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Banks, W., C. T. Greenwood & D. D. Muir, 1971. The characterization of starch and its components. Part 3. The techniques of semi-micro, differential, potentiometric iodine titration, and the factors affecting it.Staerke 23: 118–124.Google Scholar
  2. Bates, F. L., D. French & R. E. Rundle, 1943. Amylose and amylopectin contents of starches determined by their iodine complex formation.Journal of the American Chemical Society 65: 142–148.CrossRefGoogle Scholar
  3. Bruinsma, J., 1963. The quantitative analysis of chlorophylls a and b in plant extracts. In: Photochemistry and photobiology, Vol. 2. Pergamon Press Ltd., Great Britain, pp. 241–249.Google Scholar
  4. Hovenkamp-Hermelink, J. H. M., E. Jacobsen, L. P. Pijnacker, J. N. de Vries, B. Witholt & W. J. Freenstra, 1988, Cytological studies on adventitious shoots and minitubers of a monoploid potato clone.Euphytica. In press.Google Scholar
  5. Knutson, C. A., 1986. A simplified colorimetric procedure for determination of amylose in maize starches.Cereal Chemistry 63: 89–92.Google Scholar
  6. Lustinec, J., V. Hadacova, M. Kaminek & Z. Prochazka, 1983. Quantitative determiantion of starch, amylose and amylopectin in plant tissues using glass fiber paper.Analytical Biochemistry 132: 265–271.PubMedGoogle Scholar
  7. Richter, M., S. Augustat & F. Schierbaum, 1968. Bestimmung des Jodbindungsvermögens durch amperometrische Titration. In: Ausgewählte Methoden der Stärkechemie. VEB Fachbuchverlag Leipzig, pp. 126–129.Google Scholar
  8. Roest, S. & G. S. Bokelmann, 1976. Vegetative propagation ofSolanum tuberosum L. in vitro.Potato Research 19: 173–178.CrossRefGoogle Scholar
  9. Varns, J. L. & J. R. Sowokinos, 1974. A rapid micro-starch quantitation method for potato callus and its application with potato tubers.American Potato Journal 51: 383–392.Google Scholar
  10. Williams, P. C., F. D. Kuzina & I. Hlynka, 1970. A rapid colorimetric procedure for estimating the amylose content of starches and flours.Cereal Chemistry 47: 411–420.Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • J. H. M. Hovenkamp-Hermelink
    • 1
  • J. N. De Vries
    • 1
  • P. Adamse
    • 1
  • E. Jacobsen
    • 1
  • B. Witholt
    • 2
  • W. J. Feenstra
    • 1
  1. 1.Department of GeneticsUniversity of Groningen, Center of Biological SciencesHarenthe Netherlands
  2. 2.Department of BiochemistryUniversity of GroningenGroningenthe Netherlands

Personalised recommendations