Potato Research

, Volume 31, Issue 4, pp 651–658 | Cite as

A shoot induction procedure altered for increased shooting efficiency of potato protoplast-derived calli (cvs. crystal and norgold russet)

  • R. J. Taylor
  • G. A. Secor
Article
Accepted:

Summary

The responses of two potato cultivars, Crystal and Norgold Russet, to a standard protoplast regeneration procedure were examined. Both cultivars yielded protoplasts that survived and grew in the initial plating medium. Protoplast-derived callus tissue also grew on the callus proliferation medium. The cultivars differed most in their ability to survive on the shoot induction medium; changes in its growth regulator content and the quantity of agar increased shoot production of Crystal from 1% to 30% and that of Norgold Russet from 7% to 24% on modified medium under low light intensity.

Additional key words

callus culture regeneration Solanum tuberosum L 
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References

  1. Behnke, M., 1976. Kulturen isolierter Zellen von einigen dihaploidenSolanum tuberosum —Klonen und ihre Regeneration,Zeitschrift für Pflanzenphysiologie 78: 177–181.Google Scholar
  2. Bidney, D. L. & J. F. Shepard, 1981. Phenotypic variation in plants regenerated from protoplasts: the potato system.Biotechnology and Bioengineering 23: 2691–2701.CrossRefGoogle Scholar
  3. Binding, H., R. Nehls, R. Koch, J. Finger & G. Mordhorst, 1981. Comparative studies on protoplast regeneration in herbaceous species of the dicotyledoneae class.Zeitschrift für Pflanzenphysiologie 101: 119–130.Google Scholar
  4. Binding, H., R. Nehls, O. Schieder, S. K. Sopory & G. Wenzel, 1978. Regeneration of mesophyll protoplasts isolated from dihaploid clores ofSolanum tuberosum.Physiologia Plantarum 43: 52–54.Google Scholar
  5. Bokelmann, G. S. & S. Roest, 1983. Plant regeneration from protoplasts of potato (Solanum tuberosum cv. Bintje).Zeitschrift für Pflanzenphysiologie 109: 259–265.Google Scholar
  6. Cassells, A. C. & L. Tamma, 1987. Survival and division in protoplasts from tobacco (Nicotiana tabacum) depends upon the physiological state of the individual donor plant.Physiologia Plantarum 69: 317–322.Google Scholar
  7. Constantin, M. J., 1981. Chromosomal instability in cell and tissue cultures and regenerated plants.Environmental and Experimental Botany 21: 359–368.Google Scholar
  8. Foulger, D. & M. G. K. Jones, 1986. Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars.Plant Cell Reports 5: 72–76.CrossRefGoogle Scholar
  9. Fowke, L. C. & O. L. Gamborg, 1980. Applications of protoplasts to the study of plant cells.International Review of Cytology 68: 9–51.Google Scholar
  10. Grun, P. & L.-J. Chu, 1978. Development of plants from protoplasts ofSolanum (Solanaceae).American Journal of Botany 65: 538–543.Google Scholar
  11. Gunn, R. E. & J. F. Shepard, 1981. Regeneration of plants from mesophyll-derived protoplasts of British potato (Solanum tuberosum L.) cultivars.Plant Science Letters 22: 97–101.CrossRefGoogle Scholar
  12. Haberlach, G. T., B. A. Cohen, N. A. Reichert, M. A. Baer L. E. Towill & J. P. Helgeson, 1985. Isolation, culture and regeneration of protoplasts from potato and several relatedSolanum species.Plant Science 39: 67–74.CrossRefGoogle Scholar
  13. Hughes, K. W., 1981. In vitro ecology: exogenous factors affecting growth and morphogenesis in plant culture systems.Environmental and Experimental Botany 21: 281–288.CrossRefGoogle Scholar
  14. Izhar, S. & J. B. Power, 1977. Genetic studies withPetunia leaf protoplasts. I. Genetic variation to specific growth hormones and possible genetic control on stages of protoplast development in culture.Plant Science Letters 8: 375–382.CrossRefGoogle Scholar
  15. Radke, S. & P. Grun, 1986. Isolation, culture, and regeneration of leaf mesophyll protoplasts of selected clones ofSolanum.Potato Research 29: 451–462.CrossRefGoogle Scholar
  16. Schumann, U. & H. Koblitz, 1983. Studies on plant recovery from mesophyll protoplasts ofSolanum tuberosum L. andSolanum phureja Juz. et Buk.Biologia Plantarum 25: 180–186.Google Scholar
  17. Shepard, J. F., 1980a. Mutant selection and plant regeneration from potato mesophyll protoplasts. In: I. Rubenstein, B. Gengenbach, R. L. Phillips & C. E. Green (Eds), Genetic improvement of crops: Emergent techniques, pp. 185–219. Minneapolis, University of Minnesota Press.Google Scholar
  18. Shepard, J. F., 1980b. Abscisic acid-enhanced shoot initiation in protoplast-derived calli of potato.Plant Sciece Letters 18: 327–333.Google Scholar
  19. Shepard, J. F., 1981. Protoplasts as sources of disease resistance in plants.Annual Review of Phytopathology 19: 145–166.CrossRefGoogle Scholar
  20. Shepard, J. F., 1982. Cultivar dependent cultural refinements in potato protoplast regeneration.Plant Science Letters 26: 127–132.CrossRefGoogle Scholar
  21. Shepard, J. F., D. Bidney & E. Shahin, 1980. Potato protoplasts in crop improvement.Science 208: 17–24.Google Scholar
  22. Shepard, J. F. & R. E. Totten, 1977. Mesophyll cell protoplasts of potato: isolation, proliferation and plant regeneration.Plant Physiology 60: 313–316.Google Scholar
  23. Tavazza, R. & G. Ancora, 1986. Plant regeneration from mesophyll protoplasts in commerical potato cultivars (Primura, Kennebec, Spunta, Desiree).Plant Cell Reports 5: 243–246.CrossRefGoogle Scholar
  24. Thomas, E., 1981. Plant regeneration from shoot culture-derived protopasts of tetraploid potato (Solanum tuberosum cv. Maris Bard).Plant Science Letters 23: 81–88.CrossRefGoogle Scholar
  25. Thomas, E., P. J. King & I. Potrykus, 1979. Improvement of crop plants via single cells in vitro — an assessment.Zeitschrift für Pflanzenzuchtung 82: 1–30.Google Scholar
  26. Thorpe, T. A., 1980. Organogenesis in votro: structure, physiological and biochemical aspects. In: I. K. Vasil (Ed.), International review of cytology — Supplement 11B. Perspectives in plant cell tissue culture, pp. 71–111. New York, Academic Press.Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • R. J. Taylor
    • 1
  • G. A. Secor
    • 1
  1. 1.Department of Plant PathologyNorth Dakota State UniversityFargoUSA

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