Potato Research

, Volume 36, Issue 3, pp 205–217 | Cite as

Increase in phosphorylase activity during cold-induced sugar accumulation in potato tubers

  • Pieternel A. M. Claassen
  • Miriam A. W. Budde
  • Martha H. Van Calker


The accumulation of reducing sugars, sucrose and hexose phosphates in cv. Bintje and genotype KW77-2916 during storage at 2, 4, or 8°C was studied in relation to several catalytic activities. Bintje tubers accumulated sugars during storage at 2 or 4°C, whereas KW77-2916 showed reduced cold-sweetening at 2°C. The increase in glucose 6-phosphate and sucrose occurred concurrently and preceded the increase in reducing sugar concentration. Phosphorylase activity showed a strong interaction with temperature, storage duration and sugar accumulation in both genotypes. Invertase activity increased in Bintje concomitantly with the increase in reducing sugars, but this effect was less obvious in KW77-2916. The activities of other glycolytic and Krebs cycle enzymes showed no obvious correlation with sugar accumulation. It is suggested that the increase in phosphorylase activity acts as a triggering event in the sweetening of potato tubers during cold storage.

Additional keywords

Solanum tuberosum glycolytic enzymes glucose 6-phosphate 


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  1. ap Rees, T., M. M. Burrell, T. G. Entwistle, J. B. W. Hammond, D. Kirk & N. J. Kruger, 1988. Effects of low temperature on the respiratory metabolism of carbohydrates by plants. In: S. P. Long & F. I. Woodward (Eds), Plants and temperature.Symposium of the Society of Experimental Biology 42: 377–393. Comparative Biology Ltd, Cambridge.Google Scholar
  2. Barichello, V., R. Y. Yada, R. H. Coffin & D. W. Stanley, 1990. Respiratory enzyme activity in low temperature sweetening of susceptible and resistant potatoes.Journal of Food Science 55: 1060–1063.Google Scholar
  3. Barker, J., 1968. Studies in the respiratory and carbohydrate metabolism of plant tissues. XXV. Changes in rate of CO2 output and in content of various phosphate compounds in potatoes.New Phytologist 67: 495–503.Google Scholar
  4. Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein, utilizing the principle of protein-binding,Analytical Biochemistry 72: 248–254.CrossRefPubMedGoogle Scholar
  5. Bredemeijer, G. M. M., H. C. J. Burg, P. A. M. Claassen & W. J. Stiekema, 1991. Phosphofructokinase in relation to sugar accumulation in cold-stored potato tubers.Journal of Plant Physiology 138: 129–135.Google Scholar
  6. Burton, W. G., 1989. The potato. Longman Scientific & Technical, Harlow, England. p. 365–522.Google Scholar
  7. Claassen, P. A. M., M. A. W. Budde, H. J. de Ruyter, M. H. van Calker & A. van Es, 1991. Potential role of pyrophosphate: fructose 6-phosphate phosphotransferase in carbohydrate metabolism of cold stored tubers ofSolanum tuberosum cv. Bintje.Plant Physiology 95: 1243–1249.Google Scholar
  8. Colon, L. T., L. Sijpkes & K. J. Hartmans, 1989. The cold stability ofSolanum goniocalyx andS.phureja can be transferred to adapted diploid and tetraploidS.tuberosum germplasm. In: K. M. Louwes, H. A. J. M. Toussaint & L. M. W. Dellaert (Eds), Parental line breeding and selection in potato breeding. PUDOC, Wageningen. p. 76–79.Google Scholar
  9. Doehlert, D. C. & S. C. Huber, 1983. Regulation of spinach leaf sucrose phosphate synthase by glucose-6-phosphate, and Pi.Plant Physiology 73: 989–994.Google Scholar
  10. Glock, E. G. & P. McLean, 1953. Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver.Biochemical Journal 55: 400–408.PubMedGoogle Scholar
  11. Hammond, J. B. W., M. M. Burrell & N. J. Kruger, 1990. Effect of low temperature on the activity of phosphofructokinase from potato tubers.Planta 180: 613–616.Google Scholar
  12. Isherwood, F. A., 1973. Starch-sugar inversion inSolanum tuberosum.Phytochemistry 12: 2579–2591.CrossRefGoogle Scholar
  13. Isherwood, F. A., 1976. Mechanism of starch-sugar interconversion inSolanum tuberosum.Phytochemistry 15: 33–41.CrossRefGoogle Scholar
  14. Kennedy, M. G. H. & F. A. Isherwood, 1975. Activity of phosphorylase inSolanum tuberosum during low temperature storage.Phytochemistry 14: 667–670.Google Scholar
  15. Kruckeberg, A. L., H. E. Neuhaus, R. Feil, L. D. Gottlieb & M. Stitt, 1989. Decreased-activity mutants of phosphoglucose isomerase in the cytosol and chloroplast ofClarkia xantiana.Biochemical Journal 261: 457–467.PubMedGoogle Scholar
  16. Kruger, N. J., 1990. Carbohydrate synthesis and degradation. In: D. T. Dennis & D. H. Turpin (Eds), Plant physiology, biochemistry and molecular biology, Longman Group UK Ltd, Harlow. p. 59–76.Google Scholar
  17. Leszkowiat, M. J., V. Barichello, R. Y. Yada, R. H. Coffin, E. C. Lougheed & D. W. Stanley, 1990. Contribution of sucrose to nonenzymatic browning in potato chips.Journal of Food Science 55: 281–285.Google Scholar
  18. Morrell, S. & T. ap Rees, 1986. Control of the hexose content of potato tubers.Phytochemistry 25: 1073–1076.Google Scholar
  19. Ohad, I., I. Friedberg, Z. Ne'eman & M. Schramm, 1971. Biogenesis and degradation of starch. I. The fate of the amyloplast membranes during maturation and storage of potato tubers.Plant Physiology 47: 465–477.Google Scholar
  20. Pollock, C. J. & T. ap Rees, 1975. Activities of enzymes of sugar metabolism in cold-stored tubers ofSolanum tuberosum.Phytochemistry 14: 613–617.Google Scholar
  21. Pressey, R., 1969. Role of invertase in the accumulation of sugars in cold stored potatoes.American Potato Journal 46: 291–297.Google Scholar
  22. Pressey, R. & R. Shaw, 1966. Effect of temperature on invertase, invertase inhibitor, and sugars in potato tubers.Plant Physiology 41: 1657–1661.Google Scholar
  23. Richardson, D. L., H. V. Davies, H. A. Ross & G. R. Mackay, 1990. Invertase activity and its relation to hexose accumulation in potato tubers.Journal of Experimental Botany 41: 95–99.Google Scholar
  24. Sowokinos, J., 1990. Stress-induced alterations in carbohydrate metabolism. In: M. E. Vayda, & W. D. Park (Eds),The molecular and cellular biology of the potato. Biotechnology in Agriculture 3. CAB International, Wallingford. p. 137–158.Google Scholar
  25. Spychalla, J. P. & S. L. Desborough, 1990. Fatty acids, membrane permeability and sugars of stored potato tubers.Plant Physiology 94: 1207–1213.Google Scholar
  26. Trevanion, S. J. & N. J. Kruger, 1991. Effect of temperature on the kinetic properties of pyrophosphate: fructose 6-phosphate phosphotransferase from potato tuber.Journal of Plant Physiology 137: 753–759.Google Scholar
  27. Van Es, A. & K. J. Hartmans, 1987. Starch and sugars during tuberization, storage and sprouting. In: A. Rastovski & A. van Es (Eds), Storage of potatoes. PUDOC, Wageningen. p. 79–113.Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Pieternel A. M. Claassen
    • 1
  • Miriam A. W. Budde
    • 1
  • Martha H. Van Calker
    • 1
  1. 1.DLO Agrotechnological Research Institute (ATO-DLO)Wageningenthe Netherlands

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