Potato Research

, Volume 37, Issue 1, pp 25–33 | Cite as

The effect of ethylene and of inhibitors of protein and nucleic acid syntheses on dormancy break and subsequent sprout growth

  • S. M. M. Alam
  • D. P. Murr
  • L. Kristof


Cycloheximide inhibited dormancy break and subsequent sprout growth of potato buds in the presence or absence of ethylene. Dormancy break was relatively insensitive to treatment with actinomycin D, in marked contrast to the inhibition observed with cycloheximide; however, sprout elongation was inhibited by actinomycin D treatment. This suggests that dormancy release may depend on protein synthesis but not on nucleic acid synthesis.

Total protein analysis revealed that two high molecular weight polypeptide bands appeared during dormancy break in the presence or absence of ethylene. The response was consistent in the two cultivars used in this study. Cycloheximide treatment blocked the synthesis of any new polypeptides in response to ethylene in the tuber tissue. This indicates that dormancy release of potato buds may be associated with the regulation of protein synthesis.

Additional keywords

SDS-PAGE cycloheximide actinomycin tissue plug 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bruinsma, J., A. Sinnema, D. Baker & F.C. Stewart, 1967. The use of gibberellic acid (GA) and N-dimethylamino-succinamic acid (B9) in testing seed potatoes for virus infection.European Potato Journal 8: 195–203.Google Scholar
  2. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding.Analytical Biochemistry 72: 248–254.CrossRefPubMedGoogle Scholar
  3. Brady, C.J., 1988. Nucleic acid and protein synthesis, In: L.D. Nooden and A.C. Leopold (Eds), Senescence and aging in plants. Academic Press, London, U.K. pp. 147–179.Google Scholar
  4. Coleman, W.K., 1983. An evaluation of bromoethane for breaking tuber dormancy inSolanum tuberosum L.American Potato Journal 60: 161–167.Google Scholar
  5. Coleman, W.K., 1984. Large scale application of bromoethane for breaking tuber dormancy.American Potato Journal 61: 587–589.Google Scholar
  6. Coleman, W.K. & S.E. Coleman, 1986. The effects of bromoethane and ethanol on potato (Solanum tuberosum L.) tuber sprouting and subsequent yield responses.American Potato Journal 63: 373–377.Google Scholar
  7. Davern, C.I. & J. Bonner, 1958. The influence of 5-fluorouracil on tobacco mosaic virus production in tobacco leaf discs.Biochemisty Biophysics Acta 29: 205–206.Google Scholar
  8. Denny, F.E., 1926. Hastening the sprouting of dormant potato tubers.American Journal of Botany 13: 118–125.Google Scholar
  9. Dure, L. & L. Waters, 1965. Long-lived messenger RNA: Evidence from cotton seed germination.Science 147: 410–412.PubMedGoogle Scholar
  10. Edelman, J. & S.P. Singh, 1968. Studies on the biochemical basis of physiological processes in the potato tuber. Changes in nucleic acids and proteins in the sprouting tubers.Journal of Experimental Botany 19: 288–294.Google Scholar
  11. Edinoff, M., J.E. Kroll & D. Klein, 1957. Effect of 5-fluorouracil on the incorporation of precursors into nucleic acid pyrimidines.Archives of Biochemistry and Biophysics 71: 274–275.Google Scholar
  12. Hames, B.D., 1981. An Introduction to Polyacrylamide Gel Electrophoresis, In: B.D. Hames and D. Rickwood (Eds), Gel electrophoresis of proteins-a practical approach. Information Retrieval Limited Press, Oxford, U.K. pp. 61–86.Google Scholar
  13. Jacobsen, J.V., 1973. Interactions between gibberellic acid, ethylene and abscisic acid in the control of amylase synthesis in barley aleurone layers.Plant Physiology 51: 198–202.Google Scholar
  14. Keegan, A.B., K.M. Kelly, J. van Staden & M.T. Smith, 1988. The effect of ethylene on RNA and protein synthesis in germinating Mankettii (Ricinodendron rautanenii Schinz) seeds.Plant Growth Regulation 7: 227–236.Google Scholar
  15. Kroon, A.M. & H. de Vries, 1969. Antibiotics: a tool in the search for the degree of autonomy of mitochondria in higher animals.Symposium of the Society of Experimental Biology 24: 181–199.Google Scholar
  16. Lascarides, D.L., 1967. Shortening the dormant period of spring-grown seed potatoes for midsummer planting.European Potato Journal 10: 100–104.Google Scholar
  17. Lopez, T.P.J., B.L. Arreguin L. & K. Varty, 1978. The effect of ethylene on gibberellic acidinduced alpha-amylase formation in wheat aleurone layers.Plant Physiology (Supplement) 61: 111.Google Scholar
  18. Macdonald, M.M. & D.J. Osborne, 1988. Synthesis of nucleic acids and protein in tuber buds ofSolanum tuberosum during dormancy and early sprouting.Physiologia Plantarum 73: 392–400.Google Scholar
  19. Madison, M. & L. Rappaport, 1968. Regulation of bud rest in tubers of potato.Solanum tuberosum. V. Abscisic acid and inhibitors of nucleic and protein synthesis.Plant and Cell Physiology 9: 147–153.Google Scholar
  20. Meijers, C.P., 1972. Effect of carbon disulphide on the dormancy and sprouting of seed potatoes.Potato Research 15: 160–165.Google Scholar
  21. Paiva, E., 1983. Synthesis of the major potato tuber proteins and their relationship to tuberization. Ph.D. Thesis, Purdue University, 32 pp.Google Scholar
  22. Racusen, D., 1983. Occurrence of patatin during growth and storage of potato tubers.Canadian Journal of Botany 61: 370–373.Google Scholar
  23. Racusen, D. & M. Foote, 1980. A major soluble glycoprotein of potato tubers.Journal of Food Biochemistry 4: 43–52.Google Scholar
  24. Rappaport, L., S. Blumenthal-Goldschmidt, M.D. Clegg & O.E. Smith, 1965. Regulation of bud rest in tubers of potatoSolanum tuberosum L. I. Effect of growth substances on excised potato buds.Plant and Cell Physiology 6: 587–599.Google Scholar
  25. Rylski, I., L. Rappaport & H.K. Pratt, 1974. Dual effects of ethylene on potato dormancy and sprout growth.Plant Physiology, 53: 658–662.Google Scholar
  26. Siegel, M.R. & H.D. Sisler, 1964. Site of action of cycloheximide in cells ofSaccharyomyces pastorianus. I. Effect of the antibiotic on cellular metabolism.Biochemistry and Biophysics Acta 81: 70–82.Google Scholar
  27. Sisler, E. C. & C. Wood, 1988. Interactions of ethylene and CO2.Physiologia Plantarum 73: 440–444.Google Scholar
  28. Spiegel, S. & A. Marcus, 1975. Polyribosome formation in early wheat germination independent of either transcription or polyadenylation.Nature 256: 228–230.CrossRefGoogle Scholar
  29. Steel, R.G.D. & J.H. Torrie, 1980. Principles and procedures of statistics, a biometrical approach. 2nd edition. McGraw-Hill Book Co., New York, 187 pp.Google Scholar
  30. Tuan, D.Y.H. & J. Bonner, 1964. Dormancy associated with repression of genetic activity.Plant Physiology 39: 768–772.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. M. M. Alam
    • 1
  • D. P. Murr
    • 1
  • L. Kristof
    • 1
  1. 1.Department of Horticultural ScienceUniversity of GuelphGuelph, OntarioCanada

Personalised recommendations