Potato Research

, Volume 32, Issue 4, pp 463–470 | Cite as

Changes of the lipid catabolism in potato tubers from cultivars differing in susceptibility to autolysis during the storage

  • Ewa Łojkowska
  • Maria Hołubowska


The activities of lipolytic acyl-hydrolases (LAH) and lipoxygenases (LOX) were compared in stored tubers of potato cultivars resistant (Acresta, Eba, Pentland Envoy) and susceptible (Kastor, Pana, Tasso) to post-wounding autolysis. In most cultivars, LAH activities had reached a maximum by the end of December but in cv. Kastor activity continued to increase throughout the storage period. LOX activities increased during most of the storage period except in cv. Tasso. The level of the fatty acid hydroperoxides, assumed from determinations of the malonaldehyde level, also increased during storage.

Additional key words

lipolytic acyl-hydrolase lipoxygenase malonaldehyde content 


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  1. Agrawal, S., S. Banerjee & S. N. Chatterjee, 1984. Effect of oxygen on ascorbic acid-induced lipid peroxidation in liposomal membrane.Indian Journal of Biochemistry and Biophysics 21: 357–360.Google Scholar
  2. Asakawa, T. & S. Matsushita, 1979. Thiobarbituric acid test for detection lipid peroxides.Lipids 14: 401–406.Google Scholar
  3. Biley, L. J., 1967. Techniques in protein chemistry. p. 341–343. Elsevier Publication, Amsterdam.Google Scholar
  4. Berkeley, H. D. & T. Galliard, 1974. Lipids of potato tubers. IV. Effect of growth and storage on lipid degrading enzymes of potato tuber.Journal of the Science of Food and Agriculture 25 (7): 869–873.PubMedGoogle Scholar
  5. Buege, J. A. & S. D. Aust, 1978. Methods in enzymology. In: Kaplan, N. O. (Ed.), Academic Press, New York. 52: 302–310.Google Scholar
  6. Galliard, T., 1971. Enzymic deacylation of phospholipids and galactolipids in plants. Purification and properties of a lipolytic acyl hydrolase from potato tubers.Biochemical Journal 121: 379–390.PubMedGoogle Scholar
  7. Galliard, T., 1978. Lipolytic and lipoxygenase enzymes in plants and their action in wounded tissue. In: Khal, G. (Ed.), Biochemistry of wounded plant tissue. De Gruyter, Berlin, p. 155–203.Google Scholar
  8. Galliard, T., 1980. Degradation of acyl lipids: hydrolytic and oxidative enzyme. In: Stumpf, P. K. (Ed.), The biochemistry of plants. A comprehensive treatise. Academic Press, New York. 4: 85–116.Google Scholar
  9. Galliard, T. & J. A. Matthew, 1977. Lipoxygenase-mediated cleavage of fatty acids to carbonyl fragments in tomato fruits.Phytochemistry 16: 339–343.CrossRefGoogle Scholar
  10. Hasson, E. P. & G. G. Laties, 1976. Separation and characterization of potato lipid acylhydrolase.Plant Physiology 57: 142–147.Google Scholar
  11. Hidalgo, O. A. & E. Echandi, 1983. Influence of temperature and length of storage on resistance of potato to tuber rot induced byErwinia chrysanthemi.American Potato Journal 60: 1–15.Google Scholar
  12. Hiller, L. K., D. C. Koller & R. E. Thornton, 1985. Physiological disorder of potato tubers. In: Li, P. H. (Ed.), Potato physiology. Academic Press, New York, p. 389–454.Google Scholar
  13. Lesham, Y. Y., 1987. Membrane phospholipid catabolism and Ca2+ activity in control of senescence.Physiologia Plantarum 69: 551–559.Google Scholar
  14. Lesham, Y. Y., S. Sridhara & J. E. Thompson, 1984. Involvement of calcium and calmodulin in membrane detoriation senescence of pea foliage.Plant Physiology 75: 329–335.Google Scholar
  15. Lewosz, J., & E. Łojkowska, 1985. Relationship between electrolytes release from potato tubers caused by wounding or by the enzymes ofErwinia carotovora ssp.atroseptica and susceptibility to microbial decay and to mechanical damage.Ziemniak: 27–47.Google Scholar
  16. Lewosz, J., E. Łojkowska & M. Hołubowska, 1985. Physiological predisposition of potato tubers to soft rot.Ziemniak: 49–58.Google Scholar
  17. Lulai, E. C., J. R. Sowokinos & J. A. Knoper, 1986. Transluscent tissue defects inSolanum tuberosum L. II. Alternations in lipolytic acyl hydrolase, lipoxygenase, and morphology of mitochondria and amyloplasts.Plant Physiology 80: 424–428.Google Scholar
  18. Łojkowska, E., 1988. Lipid composition and post-wounding degradation in the potato slices from cultivars differing in susceptibility to autolysis.Potato Research 31: 541–549.Google Scholar
  19. Matsuda, H. & O. Hirayama, 1979. Purification and properties of a lipolytic acyl-hydrolase from potatoes leaves.Biochimica et Biophysica Acta 573: 155–165.PubMedGoogle Scholar
  20. Moreau, R. A., 1985. Membrane-degrading enzymes in the tubers of various cultivars ofSolanum tuberosum.Journal of Agricultural and Food Chemistry 33: 36–39.CrossRefGoogle Scholar
  21. Moreau, R. A. & G. Nagahashi, 1987. Glycoprotein nature of lipolytic acyl hydrolase in potato tubers and leaves. In: Stumpf, Mudd & Nes (Eds), The metabolism, structure and function of plant lipids. Plenum Press, New York & London, p. 369–371.Google Scholar
  22. Racusen, D., 1984. Lipid acyl hydrolase of patatin.Canadian Journal of Botany 62: 1640–1644.Google Scholar
  23. Van Denburgh, R. W., L. K. Hiller & D. C. Koller, 1986. Ultrastructural changes in potato tubers pith cells during brown center development.Plant Physiology 81: 167–170.Google Scholar
  24. Wardale, D. A., 1980. Lipid-degrading enzymes from potato tubers.Phytochemistry 19: 173–177.Google Scholar
  25. Weber, F. & W. Grosch, 1976. Co-oxidation of a carotenoid by the enzyme lipoxygenase: Influence of the formation of linoleic acid hydroperoxides.Zeitschrift für Lebensmittel-Untersuchung und Forschung 161: 223–230.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Ewa Łojkowska
    • 1
  • Maria Hołubowska
    • 1
  1. 1.Institute for Potato ResearchKoszalinPoland

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