Potato Research

, Volume 42, Issue 1, pp 161–171 | Cite as

Preliminary selection of antagonists ofErwinia carotovora subsp.atroseptica (Van Hall) Dye for application during green crop lifting of seed potato tubers

  • P. Kastelein
  • E. G. Schepel
  • A. Mulder
  • L. J. Turkensteen
  • J. W. L. Van Vuurde
Full Papers


Green crop lifting (GCL) for haulm killing was developed in The Netherlands and offers ideal conditions for controlling the blackleg pathogenErwinia carotovora subsp.atroseptica (Eca) by antagonists.

Based on the use of mini-tubers or young tubers from field crops, two bioassays for wound protection were developed. GCL was simulated by artificially skinning or wounding tubers, inoculating the damaged skin with Eca, treating with antagonists and incubating in either potting compost or outside in field soil.

Mainly fluorescent pseudomonads, pre-screened for in vitro antagonistic activity on agar and high soft rot reducing ability on tuber slices, were tested in the mini-tuber bioassay. Strains giving the highest degree of wound protection were further tested individually and in combination under field conditions in the young tuber bioassay. One individual strain and two combinations, resulting in reduction of contamination levels on skinned surfaces of 85% and between 60% and 70%, respectively, show good potential for biological control of blackleg.

Additional keywords

bioassay biological control blackleg green crop harvesting haulm killing skin damage wound protection Solanum tuberosum


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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • P. Kastelein
    • 1
  • E. G. Schepel
    • 2
  • A. Mulder
    • 2
  • L. J. Turkensteen
    • 1
  • J. W. L. Van Vuurde
    • 1
  1. 1.DLO-Research Institute for Plant Protection (IPO-DLO)WageningenThe Netherlands
  2. 2.H.L. Hilbrands Laboratory for Soil-borne Pests and Diseases (HLB)AssenThe Netherlands

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