Advertisement

Molecular and Cellular Biochemistry

, Volume 36, Issue 1, pp 23–27 | Cite as

Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations

  • A. R. Kornblihtt
  • M. M. Flawiá
  • D. de Mendoza
  • G. Glikin
  • R. Farías
  • H. N. Torres
Article
  • 15 Downloads

Summary

Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained binding activity for L-T3. Binding studies indicate the existence of two L-T3 receptor components associated to adenylate cyclase activity. The component exhibiting the highest hormone affinity has the lowest binding capacity.

Keywords

Sucrose Binding Activity Adenylate Cyclase Binding Capacity Purification Step 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Limbrid, L. E. & Lefkowitz, R. J., 1977. J. Biol. Chem. 252: 799–802.Google Scholar
  2. 2.
    Haga, T., Haga, K. & Gilman, A. G., 1977. J. Biol. Chem. 252:5776–5782.PubMedGoogle Scholar
  3. 3.
    Welton, A. F., Lad, P. M., Newby, A. C., Yamamura, N. Nicosia, S. & Rodbell, M., 1977. J. Biol. Chem. 252: 5947–5950.PubMedGoogle Scholar
  4. 4.
    Braun, T. & Dods, R. F., 1975. Proc. Natl. Acad. Sci. U.S.A. 72: 1097–1101.PubMedGoogle Scholar
  5. 5.
    Neer, E. J., 1978. J. Biol. Chem. 253: 5808–5812.PubMedGoogle Scholar
  6. 6.
    Flawia, M. M., Kornblihtt, A., de Mendoza, D., & Torres, H. N., 1980. Mol. and Cell. Biochem.Google Scholar
  7. 7.
    Lantham, K. R., Ring, J. C. & Baxter, J. D., 1976. J. Biol. Chem. 251: 7388–7397.Google Scholar
  8. 8.
    Worthington Enzyme Manual 1972. Worthington Biochemical Corporation: pp. 7–12, Freehold.Google Scholar
  9. 9.
    Terenzi, H. F., Roselino, E. & Passeron, S., 1971. Eur. J. Biochem. 18: 342–350.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1981

Authors and Affiliations

  • A. R. Kornblihtt
    • 1
    • 2
  • M. M. Flawiá
    • 1
    • 2
  • D. de Mendoza
    • 1
    • 2
  • G. Glikin
    • 1
    • 2
  • R. Farías
    • 1
    • 2
  • H. N. Torres
    • 1
    • 2
  1. 1.Instituto de Investigaciones Bioquímicas ‘Fundación Campomar’, Fac. de Ciencias Exactas y NaturalesUniversidad Nacional de TucumánBuenos AiresArgentina
  2. 2.Instituto de Química BiológicaUniversidad Nacional de TucumánBuenos AiresArgentina

Personalised recommendations