Journal of Orthopaedic Science

, Volume 1, Issue 3, pp 203–213 | Cite as

Anterior cruciate ligament reconstruction using iliotibial tract: Histological and mechanical studies in rabbits

  • Masayuki Minamide
  • Hideshige Moriya
  • Akihiro Tsuchiya
Original Articles

Abstract

A histological and biomechanical study of anterior cruciate ligament (ACL) reconstruction using the iliotibial tract (ITT) was carried out in rabbits. Sixty-five young adult male rabbits were used for the experiments. In the histological study, 3 weeks after the substitution, specimens, showed acellular changes, and cellular repopulation was noted from the peripheral area at 6 weeks. At 24 weeks, the original spindle-shaped fibroblasts of the substitute had changed to round cells. The ligament bone junction was formed by 48 weeks and its structure was similar to that of a normal junction. Transmission electron microscopic examination showed collagen fibril distribution in each test group. The 12-week specimens had fibrils of small diameter only, but some 24-week specimens showed a biphasic appearance, like the ACL, which has both large and small fibrils. On mechanical testing, the maximal load was decreased until 18 weeks, and then recovered to 50% of that of the normal ACL at 48 weeks, this value being approximately the same as that of the ITT used in the reconstruction.

Key words

anterior cruciate ligament reconstruction experimental study ihotibial tract histological maturation biomechanical property 

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References

  1. 1.
    Amiel D, Kleiner JB, Roux RD, et al. The phenomenon of “ligamentization”: Anterior cruciate ligament reconstruction with autogenous patellar tendon. J Orthop Res 1986;4:162–72.PubMedGoogle Scholar
  2. 2.
    Arnoczky SP, Tarvin GB, Marshall JL. Anterior cruciate ligament replacement using patellar tendon: An evaluation of graft revascularization in the dog. J Bone Joint Surg Am 1982;64:217–24.PubMedGoogle Scholar
  3. 3.
    Ballock RT, Woo SL, Lyon RM, et al. Use of patellar tendon autograft for anterior cruciate ligament reconstruction in the rabbit: A long-term histologic and biomechanical study. J Orthop Res 1989;7:474–85.CrossRefPubMedGoogle Scholar
  4. 4.
    Butler DL. Anterior cruciate ligament: Its normal response and replacement. J Orthop Res 1989;7:910–21.PubMedGoogle Scholar
  5. 5.
    Clancy WG Jr, Narechania RG, Rosenberg TD, et al. Anterior and posterior cruciate ligament reconstruction in Rhesus monkeys: A histological, microangiographic and biomechanical analysis. J Bone Joint Surg Am 1981;63:1270–84.PubMedGoogle Scholar
  6. 6.
    Cooper RR, Misol S. Tendon and ligament insertion. A light and electron microscopic study. J Bone Joint Surg Am 1970;52:1–20.PubMedGoogle Scholar
  7. 7.
    Curtis RJ, Delee JC, Drez DJ Jr. Reconstruction of the anterior cruciate ligament with freeze dried fascia lata allografts in dogs: A preliminary report. Am J Sports Med 1985;13(6):408–14.PubMedGoogle Scholar
  8. 8.
    Fulkerson JP, Berke A, Parthasarathy N. Collagen biosynthesis in rabbit intraarticular patellar tendon transplants. Am J Sports Med 1990;18:249–53.PubMedGoogle Scholar
  9. 9.
    Hart RA, Woo S.L-Y, Newton PO. Ultrastructual morphology of anterior cruciate and medial collateral ligaments: An experimental study in rabbits. J Orthop Res 1992;10:96–103.CrossRefPubMedGoogle Scholar
  10. 10.
    Hey Groves EW. The crucial ligaments of the knee joint: Their function, rupture, and the operative treatment of the same. Br J Surg 1920;7:505–15.Google Scholar
  11. 11.
    Imai N. Basic research on anterior cruciate ligament (in Japanese). J Jpn Orthop Assoc. 1960;34:43–61.Google Scholar
  12. 12.
    Kleiner JB, Amiel D, Roux RD, et al. Origin of replacement cells for the anterior cruciate ligament autograft. J Orthop Res 1986;4:466–74.CrossRefPubMedGoogle Scholar
  13. 13.
    Kondon M. An experimental study on reconstructive surgery of the anterior cruciate ligament (in Japanese). J Jpn Orthop Assoc 1979;53:521–33.Google Scholar
  14. 14.
    McIntosh DL. The anterior cruciate ligament: Over-the-top repair. Read at the Annual Meeting of the American Academy of Orthopaedic Surgeons. Dallas, Texas, 1974. J Bone Joint Surg Br 1974;56:591.Google Scholar
  15. 15.
    Makisalo SE. Collagen types I and III and fibronectin in healing anterior cruciate ligament after reconstruction with carbon fibre. Injury 1989;20(1):72–6.PubMedGoogle Scholar
  16. 16.
    Minamide M, Moriya H, Sonoda M, et al. Anterior cruciate ligament reconstruction using iliotibial tract: An experimental study in rabbits (abstract in Japanese). J Jpn Orthop Assoc 1992;66(8):S1342.Google Scholar
  17. 17.
    Minamide M, Moriya H, Tsuchiya A, et al. Anterior cruciate ligament reconstruction using iliotibial tract: A histological study in rabbits (in Japanese). J Tokyo Knee Soe 1993;14:83–6.Google Scholar
  18. 18.
    Moriya H, Takahashi K, Minamide M. Basic and clinical study on ACL reconstruction using iliotibial tract (abstract in Japanese). J Jpn Orthop Assoc 1992;66(3):S465–6.Google Scholar
  19. 19.
    Moriya H. Operative results in ACL insufficiency (in Japanese). Hiza (Knee) 1981;7(1):40–5.Google Scholar
  20. 20.
    Muneta T. The effect of exercise on the normal and reconstructed anterior cruciate ligament in rabbits (in Japanese). J Jpn Orthop Assoc 1989;63:1502–12.Google Scholar
  21. 21.
    Noyes FR, Butler DL, Grood ES. Biomechanical analysis of human ligament grafts used in knee-ligament repairs and reconstructions. J Bone Joint Surg Am 1984;66:344–52.PubMedGoogle Scholar
  22. 22.
    O'Donoghue DH, Frank GR, Jeter GL, et al. Repair and reconstruction of the anterior cruciate ligament in dogs: Factors influencing long-term results. J Bone Joint Surg Am 1971;53:710–18.PubMedGoogle Scholar
  23. 23.
    Ogata K, Cho S, Simmons DJ. Experimental study on transplanted tendon in the bone tunnel (in Japanese). Seikeigeka Kisokagaku (J Orthop Basic Science) 1985;12:232–5.Google Scholar
  24. 24.
    Otani T. Development of ligament-bone junction in anterior cruciate ligament reconstruction with the scaffold type polyester artificial ligament (Leeds-Keio) in the dog (in Japanese). J Jpn Orthop Assoc 1992;66:264–78.Google Scholar
  25. 25.
    Shino K, Kawasaki T, Hirose H, et al. Replacement of the anterior cruciate ligament by an allogenic tendon graft: An experimental study in the dog. J Bone Joint Surg Br 1984;66:672–81.PubMedGoogle Scholar
  26. 26.
    Sueyasu M. An experimental study on reconstructive surgery of anterior cruciate ligament using frec autogenous tendon (in Japanese). J Jpn Orthop Assoc 1971;45:233–41.Google Scholar
  27. 27.
    Tsuchiya A, Moriya H, Takeuchi S, et al. Problems in ACL reconstruction for young female athletes (in Japanese). J Jpn Orthop Soc Sports Med 1987;6:41–5.Google Scholar
  28. 28.
    van Rens Th JG, van den Berg AF, Huiskes R, et al. Substitution of the anterior cruciate ligament: A long-term histologic and biomechanical study with autogenous pedicled grafts of the iliotibial band in dogs. Arthroscopy 1986;2:139–54.PubMedGoogle Scholar

Copyright information

© The Japanese Orthopaedic Association 1996

Authors and Affiliations

  • Masayuki Minamide
    • 1
  • Hideshige Moriya
    • 1
  • Akihiro Tsuchiya
    • 1
  1. 1.Department of Orthopaedic Surgery, School of Medicinethe University of ChibaChibaJapan

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