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Journal of Gastroenterology

, Volume 29, Issue 6, pp 727–732 | Cite as

Expression of cytoskeletal-associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinoma

  • Shigeo Ikuta
  • Fumio Itoh
  • Yuji Hinoda
  • Minoru Toyota
  • Yusuke Makiguchi
  • Kohzoh Imai
  • Akira Yachi
Liver, Pancreas, and Biliary Tract

Abstract

We investigated the mRNA expression of cytosolic protein tyrosine phosphatase (PTPH1), which has a homologous domain to cytoskeletal-associated proteins, in human hepatocellular carcinomas (HCCs) by using reverse transcriptase-polymerase chain reaction (RT-PCR). PTPH1 mRNA was detected in all HCC cell lines (n=6), and HCC and adjacent noncancerous tissues (n=8) examined, indicating that PTPH1 was expressed in HCCs and hepatocytes. There was no remarkable difference in the level expression of PTPH1 mRNA between HCC and adjacent noncancerous tissues. We also performed RT-PCR single-strand conformation polymorphism (SSCP) analysis in HCC cell lines and tissues in the C-terminal region of the catalytic domain of PTPH1. In the cHc4 cell line and a HCC tissue specimen, a shifted band was detected, although it was not found in the noncancerous tissue of the HCC specimen. Nucleotide sequence analysis showed a common mutation from T to C at the third letter of codon 919 which did not lead to amino acid substitution. These results suggest that another mutation leading to the development of HCC could occur in some region of PTPH1 other than that investigated in this study.

Key words

protein tyrosine phosphatase PTPH1 RT-PCR RT-PCR SSCP hepatocellular carcinoma 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Shigeo Ikuta
    • 1
  • Fumio Itoh
    • 1
  • Yuji Hinoda
    • 1
  • Minoru Toyota
    • 1
  • Yusuke Makiguchi
    • 1
  • Kohzoh Imai
    • 1
  • Akira Yachi
    • 1
  1. 1.Department of Internal Medicine (Section 1), School of MedicineSapporo Medical UniversitySapporoJapan

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