Journal of Gastroenterology

, Volume 31, Issue 5, pp 639–645 | Cite as

Tissue superoxide dismutase (SOD) activity and immunohistochemical staining in acute appendicitis: Correlation with degree of inflammation

  • Akira Satomi
  • Taiju Hashimoto
  • Saburo Murakami
  • Hideaki Murai
  • Hirokazu Kawase
  • Shigeki Takahashi
  • Takao Morita
  • Moriyuki Matsuki
  • Masaru Sonoda
Alimentary Tract
Received:
Accepted:

Abstract

The mechanism of progression of appendicitis has not been clarified. We esamined tissue superoxide dismutase (SOD) activity, thiobarbituric acid reactive substance (TBARS), and the localization of Cu, Zn-SOD in 56 inflamed appendices in relation to histopathological classification. There was a significant difference in SOD activity between catarrhal appendix and phlegmonous and gangrenous appendix (2.3±0.1 vs 5.0±0.2 and 4.6±0.6 units/mg protein, respectivelyP<0.05). TBARS value was highest in gangrenous appendix, being significantly different from the levels in the other two types (0.47±0.40 vs 0.19±0.01 n mol/mg protein, in catarrhal and 0.20±0.02, in phlegmonous appendixP<0.05). Positive staining for Cu, Zn-SOD was demonstrated in 64% of catarrhal appendices, 96% of phlegmonous appendices, and 75% of gangrenous appendices, and intense positive staining was recognized in 9%, 28%, and 40% of these appendices, respectively. These results indicated that active oxygen influences the degree of inflammation in phlegmonous and gangrenous appendicitis. Gangrenous appendicitis and the other two types of appendicits seemed to be different entities.

Key words

progression of appendicitis active oxygen SOD thiobarbituric acid reactive substance 
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References

  1. 1.
    Bohrod MG: The pathogenesis of acute appendicitis. Am J. Clin Pathol 1946:16:752–760Google Scholar
  2. 2.
    Babekir ARE, Devi N. Analysis of the pathology of 405 appendicites, East Afr Med J 1990;67:599–602.PubMedGoogle Scholar
  3. 3.
    Chang AR. An analysis of the pathology of 3003 appendices. Aust NZ J Surg 1981;51:169–178.Google Scholar
  4. 4.
    Andreou P, Blain S, Du Boulay CEH. A histopathological study of the appendix at autopsy and after surgical ewsection. Histopathology 1990;117:427–431.Google Scholar
  5. 5.
    Suematsu M, Suzuki M, Kitahora T, et al. Increased respiratory burst of leucocytes in inflammatory bowel disease-analysis of free radical generation using a chemiluminescence probe. J Clin Lab Immunol 1987;24:125–128.PubMedGoogle Scholar
  6. 6.
    Kitahara T, Suzuki K, Asakura H, et al. Active oxygen species generated by monocytes and polymorphonuclear cells in Crohn's disease. Dig Dis Sci 1988;32:951–955.Google Scholar
  7. 7.
    Segal GH, Petras RE. Verniform appendix. In: Sternberg SS (ed) Histology for pathologists., New York: Raven 1992:593–905.Google Scholar
  8. 8.
    Morson BC, Dawson IMP, Morson and Dawson's gastrointestinal pathology, Oxford: Blackwell Scientific, 1989.Google Scholar
  9. 9.
    Asayama K, Uchida N, Dobashi K, et al. Immunohistochemical localization of copper zinc and manganese superoxide dismutase in murine tissues: Comparison with the results in rat. J Act Oxyg Free Red. 1991;2:122–129.Google Scholar
  10. 10.
    Nakamura S. Appendix In: Iijima S ed Current encyclopedia of pathology 12C. Tokyo: Nakayama Shoten, 1994;121–146.Google Scholar
  11. 11.
    Yamamoto K, Tasumura T, Appendicitis. In: Wada T (ed) New encyclopedia of Surgical Science 23B Tokyo: Nakayama Shoten, 1990;111–143.Google Scholar
  12. 12.
    Sonoda M, Asakuno G, Matsuki M et al. Radical trapping PBN during reperfusion in rabbit gastric mucosa. Free Rad Res Comms 1993;19:185–191.Google Scholar
  13. 13.
    Yagi K. A simple fluoremetric assay for lipid peroxide in blood plasma. Biochem Med 1976;15:212–216.CrossRefPubMedGoogle Scholar
  14. 14.
    Mashiko S, Suzuki N, Koga S et al. Measurement of rate constants for quenching singlet oxygen with a cypridian lucife-rin analog (2-methyl-6[P-methoxyphenyl]-3, 7-dihydroimidazo (1.2-a) pyrazin-3-one) and sodium azide. J Biolumin Chemilumin 1991;6:69–72.CrossRefPubMedGoogle Scholar
  15. 15.
    Yoshikawa T, Naito Y, Kondo M. The role of free radicals in the pathogenesis of human disease. J Acta Oxyg Free Rad 1990;1:83–102.Google Scholar
  16. 16.
    Rossi F. The O2-forming NADPH oxidase of the phagocytes: Nature, mechanism of activation and function. Biochem Biophys Acta 1986;853:65–89.PubMedGoogle Scholar
  17. 17.
    Aschoff L. Die Wurmforstsatzentzündung. Jena: Gustav Fischer, 1908.Google Scholar
  18. 18.
    Stone HH, Sanders SL, Martin JD. Perforated appendicitis in children. Surgery 1971;69:673–679.PubMedGoogle Scholar
  19. 19.
    Andersoon, R, Hugander A, Thulin A, et al. Indication or operation in suspected appendicitis and incidence of perforation. BMJ 1994;308:107–110.Google Scholar
  20. 20.
    Luckman R. Incidence and case fatality rates for acute appendicitis in California. Am J Epidemiol 1989;129:905–918.Google Scholar
  21. 21.
    Ambjornsson E, Bengmark S. Obstruction of the appendix lumen in relation to pathogenesis of acute appendicitis. Acta Chir Scand 1983;149:789–791.Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Akira Satomi
    • 1
  • Taiju Hashimoto
    • 1
  • Saburo Murakami
    • 1
  • Hideaki Murai
    • 1
  • Hirokazu Kawase
    • 1
  • Shigeki Takahashi
    • 1
  • Takao Morita
    • 1
  • Moriyuki Matsuki
    • 1
  • Masaru Sonoda
    • 2
  1. 1.Second Department of SurgerySaitama Medical SchoolSaitamaJapan
  2. 2.First Department of BiochemistrySaitama Medical SchoolSaitamaJapan

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